| Attributes | Values |
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| rdf:type
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| Description
| - Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs of PCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite sensitive and ab
- Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs of PCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite sensitive and ab (en)
- Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of control of D. dipsaci is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid and reliable as well as cost effective technique is needed to identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs of PCR oligonucleotides for detection and identification of the stem nematode D. dipsaci in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. The resulting two developed SCAR primer pairs were further tested using as template DNA extracted from collection of twelve healthy plant hosts, but no amplification was observed. The PCR protocol was shown to be quite sensitive and ab (cs)
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| Title
| - Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci.
- Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci. (en)
- Hledání a charkterizace SCAR markerů za účelem molekulární detekce osních nematod Ditylenchus dipsaci (cs)
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| skos:prefLabel
| - Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci.
- Identification and evaluation of SCAR markers for molecular detection of the stem and bulb nematode Ditylenchus dipsaci. (en)
- Hledání a charkterizace SCAR markerů za účelem molekulární detekce osních nematod Ditylenchus dipsaci (cs)
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| skos:notation
| - RIV/60460709:41210/06:18210!RIV07-MSM-41210___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/06:18210
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - he stem and bulb nematode, Ditylenchus dipsaci, quarantine organisms, molecular diagnostics, PCR amplification, RAPD (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...vEdiceCisloSvazku
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| http://linked.open...i/riv/nazevZdroje
| - Proceedings of XVII. Czech and Slovak Plant Protection Conference. September 12-14, 2006,
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Douda, Ondřej
- Marek, Martin
- Zouhar, Miloslav
- Ryšánek, Pavel
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Česká zemědělská univerzita v Praze
|
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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