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rdf:type
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Description
| - Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores.
- Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores. (en)
- Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores. (cs)
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Title
| - The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes
- Úloha extracelárních a intracelulárních vápníkových depozit při NO-dependentní partenogenetické aktivaci prasečích oocytů (cs)
- The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes (en)
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skos:prefLabel
| - The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes
- Úloha extracelárních a intracelulárních vápníkových depozit při NO-dependentní partenogenetické aktivaci prasečích oocytů (cs)
- The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes (en)
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skos:notation
| - RIV/60460709:41210/05:11691!RIV06-MSM-41210___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/05:11691
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - pig, oocyte, meiotic maturation, activation, calcium (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...vEdiceCisloSvazku
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http://linked.open...i/riv/nazevZdroje
| - Current Problems of Breeding, Health, Growth and Production Pigs, České Budějovice
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Petr, Jaroslav
- Ješeta, Michal
- Chmelíková, Eva
- Rozinek, Jiří
- Kuthanová, Zuzana
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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