| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - PMTV RNA 1 was divided into 6 parts with the length 1 kb aproximately, and these fragments were inserted into pUC-57A suitable for sequencing. The obtained sequences showed high homology (GenBank AY196959). All fragments were inserted into different expression vectors. First of all we used the non-tagged expression vectors, PMPM-Ω-4, 5, 6; pET-21a+ and pTH. To facilitate detection of expressed proteins we used tagged vectors - either with His tag or glutation S-transferase (GST). GST fusion protein was performed in vectors pGEX-4T-1, 2 and pGEX-KG alowing the removing of tag by thrombin. Proteins with His tag were induced in vectors pQE-40 and pQE-60. The highest yield of expressed protein was obtained from pGEX-4T-2 in E. coli BL 21. Disadvantage of this aproach was uncomplete cleavage of GST by thrombin. We consider pQE-40 and pQE-60 as more suitable vectors for the purpose of production of polyclonal antibodies against PMTV proteins, because sequence of tag 6xHis has not been found in pla
- PMTV RNA 1 was divided into 6 parts with the length 1 kb aproximately, and these fragments were inserted into pUC-57A suitable for sequencing. The obtained sequences showed high homology (GenBank AY196959). All fragments were inserted into different expression vectors. First of all we used the non-tagged expression vectors, PMPM-Ω-4, 5, 6; pET-21a+ and pTH. To facilitate detection of expressed proteins we used tagged vectors - either with His tag or glutation S-transferase (GST). GST fusion protein was performed in vectors pGEX-4T-1, 2 and pGEX-KG alowing the removing of tag by thrombin. Proteins with His tag were induced in vectors pQE-40 and pQE-60. The highest yield of expressed protein was obtained from pGEX-4T-2 in E. coli BL 21. Disadvantage of this aproach was uncomplete cleavage of GST by thrombin. We consider pQE-40 and pQE-60 as more suitable vectors for the purpose of production of polyclonal antibodies against PMTV proteins, because sequence of tag 6xHis has not been found in pla (en)
- PMTV RNA 1 was divided into 6 parts with the length 1 kb aproximately, and these fragments were inserted into pUC-57A suitable for sequencing. The obtained sequences showed high homology (GenBank AY196959). All fragments were inserted into different expression vectors. First of all we used the non-tagged expression vectors, PMPM-Ω-4, 5, 6; pET-21a+ and pTH. To facilitate detection of expressed proteins we used tagged vectors - either with His tag or glutation S-transferase (GST). GST fusion protein was performed in vectors pGEX-4T-1, 2 and pGEX-KG alowing the removing of tag by thrombin. Proteins with His tag were induced in vectors pQE-40 and pQE-60. The highest yield of expressed protein was obtained from pGEX-4T-2 in E. coli BL 21. Disadvantage of this aproach was uncomplete cleavage of GST by thrombin. We consider pQE-40 and pQE-60 as more suitable vectors for the purpose of production of polyclonal antibodies against PMTV proteins, because sequence of tag 6xHis has not been found in pla (cs)
|
| Title
| - Expression of non-structural proteins of Potato mop-top virus in procaryotic system
- Expression of non-structural proteins of Potato mop-top virus in procaryotic system (en)
- Exprese nestrukturálních proteinů mop-top viru bramboru v bakteriálním systému (cs)
|
| skos:prefLabel
| - Expression of non-structural proteins of Potato mop-top virus in procaryotic system
- Expression of non-structural proteins of Potato mop-top virus in procaryotic system (en)
- Exprese nestrukturálních proteinů mop-top viru bramboru v bakteriálním systému (cs)
|
| skos:notation
| - RIV/60460709:41210/04:8778!RIV/2005/MSM/412105/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/04:8778
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - PMTV, fusion proteins, exprimation, antibodies (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...v/mistoKonaniAkce
| |
| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| - Proceeding of the XVI. Slovak and Czech Plant Protection Conference
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Čeřovská, Noemi
- Ryšánek, Pavel
- Pečenková, Tamara
- Rosecká, Pavla
|
| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Slovenská poľnohospodárska univerzita v Nitre
|
| http://localhost/t...ganizacniJednotka
| |
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