| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes were blocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from metaphase I.
- The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes were blocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from metaphase I. (en)
- The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes were blocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from metaphase I. (cs)
|
| Title
| - Účinek inhibitoru proteazómu MG132 na meiotické zrání prasečích oocytů (cs)
- Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes
- Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes (en)
|
| skos:prefLabel
| - Účinek inhibitoru proteazómu MG132 na meiotické zrání prasečích oocytů (cs)
- Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes
- Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes (en)
|
| skos:notation
| - RIV/60460709:41210/04:7180!RIV/2005/MSM/412105/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60460709:41210/04:7180
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Maturation, MG132, Oocyte, Pig, Proteasome (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Jílek, František
- Petr, Jaroslav
- Rajmon, Radko
- Chmelíková, Eva
- Sedmíková, Markéta
- Švestková, Dana
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)