About: Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0,02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to thestage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. The amount of calcium deposits in the oocyte and granulose cells increased during oocyte meiotic maturation in vitro, especially in the nucleus, mitochondria , vacules, and cytoplasm. The replenishment of calcium deposits is significantly changed under the effect of verapamil. The increase of calcium deposits in the oocyte nucleus was dalayed. A much larger amount of deposits was demonstratively smaller. A significant peak in the accumulation of calcium deposits was observed in the cytoplasm of verapamil-treated oocytes after 16 hours of in vitro culture. We propose that an altered pattern in the replenishment of calcium deposits can disturb intracellular si The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0,02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to thestage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. The amount of calcium deposits in the oocyte and granulose cells increased during oocyte meiotic maturation in vitro, especially in the nucleus, mitochondria , vacules, and cytoplasm. The replenishment of calcium deposits is significantly changed under the effect of verapamil. The increase of calcium deposits in the oocyte nucleus was dalayed. A much larger amount of deposits was demonstratively smaller. A significant peak in the accumulation of calcium deposits was observed in the cytoplasm of verapamil-treated oocytes after 16 hours of in vitro culture. We propose that an altered pattern in the replenishment of calcium deposits can disturb intracellular si (en)
Title	Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil (en)
skos:prefLabel	Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil (en)
skos:notation	RIV/60460709:41210/03:00005585!RIV/2004/MSM/412104/N
http://linked.open.../vavai/riv/strany	253-260
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 412100003)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Rozinek, Jiří Jílek, František Rajmon, Radko Sedmíková, Markéta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta agrobiologie, potravinových a přírodních zdrojů
http://linked.open...dnocenehoVysledku	632000
http://linked.open...ai/riv/idVysledku	RIV/60460709:41210/03:00005585
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	oocyte, calcium deposits, maturation in vitro, pig, verapamil (en)
http://linked.open.../riv/klicoveSlovo	oocyte pig calcium deposits verapamil maturation in vitro
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[9BA71D5125D3]
http://linked.open...i/riv/nazevZdroje	Zygote
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Jílek, František Petr, Jaroslav Rajmon, Radko Vaňourková, Z. Lánská, Vilma Rozinek, Jiří Sedmíková, Marketa
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20412100003
issn	0967-1994
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	41210

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