| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Biogenní aminy pocházejí ze surovin a fermentačních fází výroby piva. Tyto látky jsou toxické :jejich obsah by neměl překročit hygienické limity, a proto jsou pečlivě kontrolovány. Zejména pro histamin, tyramin, tryptamin, putrescin a kadaverin byla vypracován postup pro rutinní analýzu metodou HPLC s fluorescenční detekcí (ex = 330 nm, em = 440 nm) a automatickou předkolonovou derivatizací s ortho-phthaldehydem v prostředí borátového pufru. Aminy byly izolovány ze vzorků piva na nové stacionární fázi Discovery DSC-MCAX a po čistícím kroku byly vymyty alkalizovaným methanolem (1% NaOH). 1,7-diaminoheptan byl použit jako vnitřní standard pro kontrolu derivatizačního procesu. Jednoduchá příprava vzorku a automatický proces HPLC systému umožňují rychlé stanovení biogenních aminů v pivu. (cs)
- The biogenic amines in beer originate from the raw materials and the fermentation phases of beer production. These compounds are toxic: their contents should not exceed hygienic limits and are therefore carefully controlled. Predominantly histamine, tyramine, tryptamine, putrescine and cadaverine were routinely analysed by HPLC with fluorescence detection (ex = 330 nm, em = 440 nm) and an automatic precolunm derivatization with ortho-phthaldehyde in the presence of a borate buffer. The amines were isolated from beer samples in the new stationary phase Discovery DSC-MCAX and after a clean–up procedure were washed with alkalized methanol (1% NaOH). 1,7-diaminoheptan was used as an internal standard to check the derivatization procedure. Easy sample preparation and the automatic operating HPLC system enable a speedy determination of biogenic amines in beer.
- The biogenic amines in beer originate from the raw materials and the fermentation phases of beer production. These compounds are toxic: their contents should not exceed hygienic limits and are therefore carefully controlled. Predominantly histamine, tyramine, tryptamine, putrescine and cadaverine were routinely analysed by HPLC with fluorescence detection (ex = 330 nm, em = 440 nm) and an automatic precolunm derivatization with ortho-phthaldehyde in the presence of a borate buffer. The amines were isolated from beer samples in the new stationary phase Discovery DSC-MCAX and after a clean–up procedure were washed with alkalized methanol (1% NaOH). 1,7-diaminoheptan was used as an internal standard to check the derivatization procedure. Easy sample preparation and the automatic operating HPLC system enable a speedy determination of biogenic amines in beer. (en)
|
| Title
| - The HPLC determination of biogenic amines in beer by solid phase extraction (SPE) and automatic precolumn derivatization
- The HPLC determination of biogenic amines in beer by solid phase extraction (SPE) and automatic precolumn derivatization (en)
- HPLC stanovení biogenních amínů v pivu pomocí extrakce na pevné fázi (SPE) a automatické předkolonové derivatizace (cs)
|
| skos:prefLabel
| - The HPLC determination of biogenic amines in beer by solid phase extraction (SPE) and automatic precolumn derivatization
- The HPLC determination of biogenic amines in beer by solid phase extraction (SPE) and automatic precolumn derivatization (en)
- HPLC stanovení biogenních amínů v pivu pomocí extrakce na pevné fázi (SPE) a automatické předkolonové derivatizace (cs)
|
| skos:notation
| - RIV/60193697:_____/05:#0000088!RIV06-MSM-60193697
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60193697:_____/05:#0000088
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - biogenic amines; histamine; tyramine; traptamine; putrescine; cadavere; HPLC; fluorescence detection; SPE clean up (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...v/mistoKonaniAkce
| |
| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| - Proceedings of the 30th EBC Congress Prague 2005
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Horák, Tomáš
- Kellner, Vladimír
- Čejka, Pavel
- Jurková, Marie
- Čulík, Jiří
|
| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| |
| https://schema.org/isbn
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)