About: Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype

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About: Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/pmic.201400198/abstract;jsessionid=E6D0AB6647D589B559F50338BD786CD5.f02t02
Description	The posttranscriptional regulatory protein Hfq was shown to be an important determinant of the stress resistance and full virulence in the dangerous human pathogen Francisella tularensis. Transcriptomics brought rather limited clues to the precise contribution of Hfq in virulence. To reveal the molecular basis of the attenuation caused by hfq inactivation, we employed iTRAQ in the present study and compared proteomes of the parent and isogenic Delta hfq strains. We show that Hfq modulates the level of 76 proteins. Most of them show decreased abundance in the Delta hfq mutant, thereby indicating that Hfq widely acts rather as a positive regulator of Francisella gene expression. Several key Francisella virulence factors including those encoded within the Francisella pathogenicity island were found among the downregulated proteins, which is in a good agreement with the attenuated phenotype of the Delta hfq strain. To further validate the iTRAQ exploratory findings, we subsequently performed targeted LC-SRM analysis of selected proteins. This accurate quantification method corroborated the trends found in the iTRAQ data. The posttranscriptional regulatory protein Hfq was shown to be an important determinant of the stress resistance and full virulence in the dangerous human pathogen Francisella tularensis. Transcriptomics brought rather limited clues to the precise contribution of Hfq in virulence. To reveal the molecular basis of the attenuation caused by hfq inactivation, we employed iTRAQ in the present study and compared proteomes of the parent and isogenic Delta hfq strains. We show that Hfq modulates the level of 76 proteins. Most of them show decreased abundance in the Delta hfq mutant, thereby indicating that Hfq widely acts rather as a positive regulator of Francisella gene expression. Several key Francisella virulence factors including those encoded within the Francisella pathogenicity island were found among the downregulated proteins, which is in a good agreement with the attenuated phenotype of the Delta hfq strain. To further validate the iTRAQ exploratory findings, we subsequently performed targeted LC-SRM analysis of selected proteins. This accurate quantification method corroborated the trends found in the iTRAQ data. (en)
Title	Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype (en)
skos:prefLabel	Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype (en)
skos:notation	RIV/60162694:G44__/14:43875204!RIV15-MO0-G44_____
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	21-22
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Dresler, Jiří Lenčo, Juraj Stulík, Jiří Klimentová, Jana Link, Marek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	6813
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/14:43875204
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Virulence; Microbiology; Mass spectrometry; Hfq; Francisella tularensis (en)
http://linked.open.../riv/klicoveSlovo	Mass spectrometry Francisella tularensis Hfq Microbiology Virulence
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[285FF8DE8E50]
http://linked.open...i/riv/nazevZdroje	Proteomics
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Dresler, Jiří Klimentová, Jana Lenčo, Juraj Stulík, Jiří Tambor, Vojtěch Charbit, Alain Link, Marek Peterek, Miroslav
http://linked.open...ain/vavai/riv/wos	000344807600003
issn	1615-9853
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/pmic.201400198
http://localhost/t...ganizacniJednotka	G44

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