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| Description
| - The role of antibodies in the course of Francisella tularensis infection is still a subject of debate. In this study, we demonstrate that passive transfer of immune sera protected irradiated mice against subsequent infection with attenuated as well as virulent strains of F. tularensis subsp. holarctica. Naïve mice of BALB/c or C3H/CBi strains were subjected to passive transfer of sera obtained from immunized mice with live F. tularensis LVS, F. tularensis subsp. holarctica strain 15, heat-killed F. tularensis LVS, or heat killed strain 15 two hours before infection with lethal doses of LVS or strain 15. The passive transfer of sera obtained from immunized mice conferred full protection of naïve unirradiated as well as sublethally irradiated mice against low lethal doses of infection with F. tularensis LVS or strain 15, in all variants of the experiments. Moreover, the passively protected mice that survived the primary infection with F. tularensis LVS were protected also against further secondary challenge with a highly virulent strain of F. tularensis subsp. tularensis (SchuS4). Using a general immunoproteomic approach, we also identified and characterized the repertoire of F. tularensis LVS proteins recognized by anti-LVS antisera used for these transfer studies. Collectively, these results aid in finding potential target molecules that can activate the host immune system, and demonstrate a successful strategy for immunizing immunocompromised animals.
- The role of antibodies in the course of Francisella tularensis infection is still a subject of debate. In this study, we demonstrate that passive transfer of immune sera protected irradiated mice against subsequent infection with attenuated as well as virulent strains of F. tularensis subsp. holarctica. Naïve mice of BALB/c or C3H/CBi strains were subjected to passive transfer of sera obtained from immunized mice with live F. tularensis LVS, F. tularensis subsp. holarctica strain 15, heat-killed F. tularensis LVS, or heat killed strain 15 two hours before infection with lethal doses of LVS or strain 15. The passive transfer of sera obtained from immunized mice conferred full protection of naïve unirradiated as well as sublethally irradiated mice against low lethal doses of infection with F. tularensis LVS or strain 15, in all variants of the experiments. Moreover, the passively protected mice that survived the primary infection with F. tularensis LVS were protected also against further secondary challenge with a highly virulent strain of F. tularensis subsp. tularensis (SchuS4). Using a general immunoproteomic approach, we also identified and characterized the repertoire of F. tularensis LVS proteins recognized by anti-LVS antisera used for these transfer studies. Collectively, these results aid in finding potential target molecules that can activate the host immune system, and demonstrate a successful strategy for immunizing immunocompromised animals. (en)
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| Title
| - Specific antibodies protect gamma-irradiated mice against Francisella tularensis infection
- Specific antibodies protect gamma-irradiated mice against Francisella tularensis infection (en)
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| skos:prefLabel
| - Specific antibodies protect gamma-irradiated mice against Francisella tularensis infection
- Specific antibodies protect gamma-irradiated mice against Francisella tularensis infection (en)
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| skos:notation
| - RIV/60162694:G44__/12:43873094!RIV13-MO0-G44_____
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(GAP302/11/1631), P(NS9747)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60162694:G44__/12:43873094
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - F. tularensis, specific antibodies, protection, gamma irradiation, cytokines (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Balonová, Lucie
- Kročová, Zuzana
- Pejchal, Jaroslav
- Stulík, Jiří
- Kubelková, Klára
- Macela, Aleš
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1016/j.micpath.2012.07.006
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| http://localhost/t...ganizacniJednotka
| |
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