About: Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning

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About: Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bioscavengers are considered as promising antidotes against organophosphate poisoning. We focused on a bacterial phosphotriesterase (PTE) expressed in Escherichia coli. The main disadvantage of this non-human catalytic bioscavenger is its relatively short half-life in the organism and strong immunogenicity after repeated administration. Therefore, we prepared different methoxy polyethylene glycol (MPEG) conjugated recombinant PTE as a potential catalytic bioscavenger with the aim to improve its biological properties. Enzyme was modified with two linear monofunctional MPEG derivatives with reactive aldehyde group of molecular weight 2 kDa and 5 kDa. We optimized reaction conditions (reagent ratios, temperature and duration of modification reaction) and we prepared homogeneous population of fully modified recombinant PTE with molecular weight around 52 kDa and 76 kDa, respectively. Modified PTE was characterized using SDS-PAGE and MALDI-TOF and by determining K-m and V-max. Bioscavengers are considered as promising antidotes against organophosphate poisoning. We focused on a bacterial phosphotriesterase (PTE) expressed in Escherichia coli. The main disadvantage of this non-human catalytic bioscavenger is its relatively short half-life in the organism and strong immunogenicity after repeated administration. Therefore, we prepared different methoxy polyethylene glycol (MPEG) conjugated recombinant PTE as a potential catalytic bioscavenger with the aim to improve its biological properties. Enzyme was modified with two linear monofunctional MPEG derivatives with reactive aldehyde group of molecular weight 2 kDa and 5 kDa. We optimized reaction conditions (reagent ratios, temperature and duration of modification reaction) and we prepared homogeneous population of fully modified recombinant PTE with molecular weight around 52 kDa and 76 kDa, respectively. Modified PTE was characterized using SDS-PAGE and MALDI-TOF and by determining K-m and V-max. (en)
Title	Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning (en)
skos:prefLabel	Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning (en)
skos:notation	RIV/60162694:G44__/10:00002374!RIV11-MO0-G44_____
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(IAA603020901), V, Z(MO0FVZ0000604)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Link, Marek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	281334
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/10:00002374
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	phosphotriesterase; acetylcholinesterase; butyrylcholinesterase; organophosphate; scavenger; bioscavenger (en)
http://linked.open.../riv/klicoveSlovo	organophosphate scavenger acetylcholinesterase butyrylcholinesterase bioscavenger phosphotriesterase
http://linked.open...odStatuVydavatele	IE - Irsko
http://linked.open...ontrolniKodProRIV	[F390C739A8C2]
http://linked.open...i/riv/nazevZdroje	Chemico-Biological Interactions
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Actinomycete and fungi contribution to the decomposition of plant litter in the ecosystems contaminated with heavy metals.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	187
http://linked.open...iv/tvurceVysledku	Jun, Daniel Musilová, Lucie Masson, Patrick Nachon, Florian Rochu, Daniel Link, Marek Loiodice, Melanie Renault, Frederique
http://linked.open...ain/vavai/riv/wos	000281108600069
http://linked.open...n/vavai/riv/zamer	Informační zabezpečení krizového managementu pro oblast zdravotnictví
issn	0009-2797
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	G44

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