About: Proteome analysis of an attenuated francisella tularensis dsbA mutant: identification of potential dsbA substrate proteins

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About: Proteome analysis of an attenuated francisella tularensis dsbA mutant: identification of potential dsbA substrate proteins Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Francisella tularensis (F. tularensis) is highly infectious for humans via aerosol route and untreated infections with the highly virulent subsp. tularensis can be fatal. Our knowledge regarding key virulence determinants has increased recently but is still somewhat limited. Surface proteins are potential virulence factors and therapeutic targets, and in this study, we decided to target three genes encoding putative membrane lipoproteins in F. tularensis LVS. One of the genes encoded a protein with high homology to the protein family of disulfide oxidoreductases DsbA. The two other genes encoded proteins with homology to the VacJ, a virulence determinant of Shigella flexneri. The gene encoding the DsbA homologue was verified to be required for survival and replication in macrophages and importantly also for in vivo virulence in the mouse infection model for tularemia. Using a combination of classical and shotgun proteome analyses, we were able to identify several proteins that accumulated in fractions Francisella tularensis (F. tularensis) is highly infectious for humans via aerosol route and untreated infections with the highly virulent subsp. tularensis can be fatal. Our knowledge regarding key virulence determinants has increased recently but is still somewhat limited. Surface proteins are potential virulence factors and therapeutic targets, and in this study, we decided to target three genes encoding putative membrane lipoproteins in F. tularensis LVS. One of the genes encoded a protein with high homology to the protein family of disulfide oxidoreductases DsbA. The two other genes encoded proteins with homology to the VacJ, a virulence determinant of Shigella flexneri. The gene encoding the DsbA homologue was verified to be required for survival and replication in macrophages and importantly also for in vivo virulence in the mouse infection model for tularemia. Using a combination of classical and shotgun proteome analyses, we were able to identify several proteins that accumulated in fractions (en)
Title	Proteome analysis of an attenuated francisella tularensis dsbA mutant: identification of potential dsbA substrate proteins Proteome analysis of an attenuated francisella tularensis dsbA mutant: identification of potential dsbA substrate proteins (en)
skos:prefLabel	Proteome analysis of an attenuated francisella tularensis dsbA mutant: identification of potential dsbA substrate proteins Proteome analysis of an attenuated francisella tularensis dsbA mutant: identification of potential dsbA substrate proteins (en)
skos:notation	RIV/60162694:G44__/09:00002208!RIV10-GA0-G44_____
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA310/07/0226), P(GP310/06/P266), P(OC 151)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kročová, Zuzana Stulík, Jiří Klimentová, Jana Pávková, Ivona Link, Marek Strašková, Adéla Kroča, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	337275
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/09:00002208
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Francisella tularensis; virulence factor; DsbA; VacJ; lipoproteins; oxidoreductase activity; 2-DE; shotgun proteomics (en)
http://linked.open.../riv/klicoveSlovo	shotgun proteomics Francisella tularensis virulence factor DsbA lipoproteins 2-DE oxidoreductase activity VacJ
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F628B1AA8DC2]
http://linked.open...i/riv/nazevZdroje	Journal of Proteome Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20151 Proteomic analysis Francisella tularensis LVS cultivated intracellular in vitro and in vivo, identification of potential virulence factors Study of B-cell interaction with intracellular pathogen Francisella tularensis.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Klimentová, Jana Kročová, Zuzana Pávková, Ivona Stulík, Jiří Fučíková, Alena Link, Marek Forslund, Anna-Lena Strašková, Adéla Kroča, Michal Forsberg, Ake Kuoppa, Kerstin Noppa, Laila
issn	1535-3893
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	G44

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