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  • We produced the mouse monoclonal and polyclonal antibodies for the detection of intracellular pathogen and potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodies and 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbent assay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4x106 CFU/ml for polyclonal antibodies and 6.9x106 CFU/ml for monoclonal antibodies. The value sample can be distinguished from any concentration of another gram-negative bacterium: Escherichia coli.
  • We produced the mouse monoclonal and polyclonal antibodies for the detection of intracellular pathogen and potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodies and 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbent assay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4x106 CFU/ml for polyclonal antibodies and 6.9x106 CFU/ml for monoclonal antibodies. The value sample can be distinguished from any concentration of another gram-negative bacterium: Escherichia coli. (en)
  • Byla vyprodukována monoklonální a polyklonální protilátka vhodná pro detekci intracelulárního parazita a potenciální BWA Francisella tularensis. Titr protilátek byl 1:640 pro polyklonální a 1:320 pro monoklonální protilátku. Obě protilátky byly použity pro nepřímou metodu ELISA tak aby byly detekovány celé buňky F. tularensis. Limit detekce byl 5.4x106 CFU/ml pro polyklonální protilátku a 6.9x106 CFU/ml pro monoklonální. Pozitivní detekci bylo možné odlišit od kontroly tvořené jinou gram-negativní bakterií: Escherichia coli. (cs)
Title
  • ELISA detection of Francisella tularensis using polyclonal and monoclonal antibodies
  • Detekce mikroorganizmu Francisella tularensis metodou ELISA za použití polyklonálních a monoklonálních protilátek (cs)
  • ELISA detection of Francisella tularensis using polyclonal and monoclonal antibodies (en)
skos:prefLabel
  • ELISA detection of Francisella tularensis using polyclonal and monoclonal antibodies
  • Detekce mikroorganizmu Francisella tularensis metodou ELISA za použití polyklonálních a monoklonálních protilátek (cs)
  • ELISA detection of Francisella tularensis using polyclonal and monoclonal antibodies (en)
skos:notation
  • RIV/60162694:G44__/08:00002027!RIV09-MO0-G44_____
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MO0FVZ0000604)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
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  • 365997
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  • RIV/60162694:G44__/08:00002027
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  • tularemia; Francisella tularensis; ELISA; biowarfare agent; immunodetection; enzyme-linked immunosorbent assay (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • IN - Indická republika
http://linked.open...ontrolniKodProRIV
  • [F0A2B9F3488D]
http://linked.open...i/riv/nazevZdroje
  • Defence Science Journal
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  • 58
http://linked.open...iv/tvurceVysledku
  • Pohanka, Miroslav
  • Pavliš, Oto
  • Kroča, Michal
http://linked.open...ain/vavai/riv/wos
  • 000259597500012
http://linked.open...n/vavai/riv/zamer
issn
  • 0011-748X
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  • G44
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