About: Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Wheat belongs to six major food allergens inducing IgE-mediated hypersensitivity reaction manifesting as cutaneous, gastrointestinal, and respiratory symptoms. Although cereals are a staple food item in most diets, only a few wheat proteins causing hypersensitivity have been identified. To characterize wheat allergens, salt-soluble wheat extracts were separated by 1-DE and 2-DE and IgE-binding proteins were detected by immunoblotting using sera of patients with allergy to ingested wheat. Proteins, frequently recognized by IgE on 2-DE were analyzed by MALDI-TOF and QTOF and their spectrum was completed by 1-DE and LCQ(DECA) nLC-MS/MS IT technique. Using all three techniques we identified 19 potential wheat allergens such as alpha-amylase inhibitors, beta-amylase, profilin, serpin, beta-D-glucan exohydrolase, and 27K protein. Employing newly developed ELISA, levels of IgE Abs against Sulamit wheat extract and alpha-amylase inhibitors type 1 and 3 were quantified and shown to be significantly elevated in Wheat belongs to six major food allergens inducing IgE-mediated hypersensitivity reaction manifesting as cutaneous, gastrointestinal, and respiratory symptoms. Although cereals are a staple food item in most diets, only a few wheat proteins causing hypersensitivity have been identified. To characterize wheat allergens, salt-soluble wheat extracts were separated by 1-DE and 2-DE and IgE-binding proteins were detected by immunoblotting using sera of patients with allergy to ingested wheat. Proteins, frequently recognized by IgE on 2-DE were analyzed by MALDI-TOF and QTOF and their spectrum was completed by 1-DE and LCQ(DECA) nLC-MS/MS IT technique. Using all three techniques we identified 19 potential wheat allergens such as alpha-amylase inhibitors, beta-amylase, profilin, serpin, beta-D-glucan exohydrolase, and 27K protein. Employing newly developed ELISA, levels of IgE Abs against Sulamit wheat extract and alpha-amylase inhibitors type 1 and 3 were quantified and shown to be significantly elevated in (en) Pšenice patří mezi 6 hlavních potravinových alergenů vyvolávající hypersenzitivní reakci zprostředkovanou IgE protilátkami. V této studii bylo identifikováno 19 pšeničních antigenů, které byly rozpoznávány IgE protilátkami od séry pacientů s alergií na pšeničné antigeny. (cs)
Title	Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients Proteomická analýza pšeničných proteinů rozpoznávaná IgE protilátkami alergických pacientů (cs) Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients (en)
skos:prefLabel	Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients Proteomická analýza pšeničných proteinů rozpoznávaná IgE protilátkami alergických pacientů (cs) Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients (en)
skos:notation	RIV/60162694:G44__/08:00002011!RIV09-MO0-G44_____
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1QS500200572), P(2B06155), P(GA310/05/2245), P(GA310/07/0414), P(GD310/03/H147), P(IAA500200801), P(KJB5020407), P(LC07017), Z(AV0Z50200510), Z(MO0FVZ0000501)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Hernychová, Lenka Stulík, Jiří Hubálek, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	390642
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/08:00002011
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Basophil activation; IgE antibodies; wheat allergens (en)
http://linked.open.../riv/klicoveSlovo	Basophil activation wheat allergens IgE antibodies
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D1347675F2F2]
http://linked.open...i/riv/nazevZdroje	Proteomics
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of new tools for diagnosis and therapy of CrohnŮs disease and ulcerative colitis Characteristic features of immune response to wheat proteins in allergic and celiac patients Regulatory mechanisms of the immune responses and their potential for development of new preventive and therapeutic strategies Identification of new markers for molecular diagnosis of inflammatory and tumor diseases. The role of innate and adaptive immunity in development of gluten-sensitive entheropathy Food allergy to wheat proteins Oncoproteomics Centre The role of the innate immunity in the development of celiac disease and the food allergy
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Hernychová, Lenka Hubálek, Martin Novák, Petr Stulík, Jiří Tučková, Ludmila Šotkovský, Petr Fuchs, Milan Havranová, Milada Kitanovicová, Alena Šetinová, Iva
http://linked.open...ain/vavai/riv/wos	000255314300013
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Zdravotnická problematika ZHN
issn	1615-9853
number of pages	15 (xsd:int)
http://localhost/t...ganizacniJednotka	G44

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