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rdf:type
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Description
| - Wheat belongs to six major food allergens inducing IgE-mediated hypersensitivity reaction manifesting as cutaneous, gastrointestinal, and respiratory symptoms. Although cereals are a staple food item in most diets, only a few wheat proteins causing hypersensitivity have been identified. To characterize wheat allergens, salt-soluble wheat extracts were separated by 1-DE and 2-DE and IgE-binding proteins were detected by immunoblotting using sera of patients with allergy to ingested wheat. Proteins, frequently recognized by IgE on 2-DE were analyzed by MALDI-TOF and QTOF and their spectrum was completed by 1-DE and LCQ(DECA) nLC-MS/MS IT technique. Using all three techniques we identified 19 potential wheat allergens such as alpha-amylase inhibitors, beta-amylase, profilin, serpin, beta-D-glucan exohydrolase, and 27K protein. Employing newly developed ELISA, levels of IgE Abs against Sulamit wheat extract and alpha-amylase inhibitors type 1 and 3 were quantified and shown to be significantly elevated in
- Wheat belongs to six major food allergens inducing IgE-mediated hypersensitivity reaction manifesting as cutaneous, gastrointestinal, and respiratory symptoms. Although cereals are a staple food item in most diets, only a few wheat proteins causing hypersensitivity have been identified. To characterize wheat allergens, salt-soluble wheat extracts were separated by 1-DE and 2-DE and IgE-binding proteins were detected by immunoblotting using sera of patients with allergy to ingested wheat. Proteins, frequently recognized by IgE on 2-DE were analyzed by MALDI-TOF and QTOF and their spectrum was completed by 1-DE and LCQ(DECA) nLC-MS/MS IT technique. Using all three techniques we identified 19 potential wheat allergens such as alpha-amylase inhibitors, beta-amylase, profilin, serpin, beta-D-glucan exohydrolase, and 27K protein. Employing newly developed ELISA, levels of IgE Abs against Sulamit wheat extract and alpha-amylase inhibitors type 1 and 3 were quantified and shown to be significantly elevated in (en)
- Pšenice patří mezi 6 hlavních potravinových alergenů vyvolávající hypersenzitivní reakci zprostředkovanou IgE protilátkami. V této studii bylo identifikováno 19 pšeničních antigenů, které byly rozpoznávány IgE protilátkami od séry pacientů s alergií na pšeničné antigeny. (cs)
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Title
| - Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients
- Proteomická analýza pšeničných proteinů rozpoznávaná IgE protilátkami alergických pacientů (cs)
- Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients (en)
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skos:prefLabel
| - Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients
- Proteomická analýza pšeničných proteinů rozpoznávaná IgE protilátkami alergických pacientů (cs)
- Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients (en)
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skos:notation
| - RIV/60162694:G44__/08:00002011!RIV09-MO0-G44_____
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1QS500200572), P(2B06155), P(GA310/05/2245), P(GA310/07/0414), P(GD310/03/H147), P(IAA500200801), P(KJB5020407), P(LC07017), Z(AV0Z50200510), Z(MO0FVZ0000501)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60162694:G44__/08:00002011
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Basophil activation; IgE antibodies; wheat allergens (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hernychová, Lenka
- Hubálek, Martin
- Novák, Petr
- Stulík, Jiří
- Tučková, Ludmila
- Šotkovský, Petr
- Fuchs, Milan
- Havranová, Milada
- Kitanovicová, Alena
- Šetinová, Iva
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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