About: Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry

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About: Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Specific markers for Coxiella burnetii (C.b.) isolates RSA 493, Priscilla, and BUD were detected using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). The method revealed noticeable differences in the ion signal profiles of the isolates in the mass range of 318 kDa. The number of characteristic ions for RSA 493, BUD, and Priscilla was 24, 15, and 7, respectively. The specific markers were compared against C.b. database using the Tag-Ident proteomics tool. For the isolates RSA 493, Priscilla and BUD there were identified 11, 5 and 3 potential biomarkers, respectively. This method represents a powerful tool for the rapid, sensitive, and differential characterization of C.b. isolates and is a good candidate for phyloproteomic approaches. Specific markers for Coxiella burnetii (C.b.) isolates RSA 493, Priscilla, and BUD were detected using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). The method revealed noticeable differences in the ion signal profiles of the isolates in the mass range of 318 kDa. The number of characteristic ions for RSA 493, BUD, and Priscilla was 24, 15, and 7, respectively. The specific markers were compared against C.b. database using the Tag-Ident proteomics tool. For the isolates RSA 493, Priscilla and BUD there were identified 11, 5 and 3 potential biomarkers, respectively. This method represents a powerful tool for the rapid, sensitive, and differential characterization of C.b. isolates and is a good candidate for phyloproteomic approaches. (en) Specifické C. burnetii márkery izolátů RSA 493, Priscilla, a BUD byly detekovány MALDI-TOF hmotnostní spekrometrií. Metoda odhalila nápadné rozdíly v profilech iontových signálů v hmotnostním rozsahu 318 kDa. Počet charakteristických iontů pro kmeny RSA 493, BUD a Priscilla byly 24, 15 a 7. Specifické márkery byly porovnány proti C. burnetii databázi použitím Tag-Ident proteomickým nástrojem. Pro izoláty RSA 493, Priscilla a BUD byly identifikováno 11, 5 a 3 potenciální biomárkery. Tato metoda představuje výkonný nástroj pro rychlou, citlivou a rozlišující charakterizaci izolátů C. burnetii a vhodné kandidáty pro fyloproteomické přístupy. (cs)
Title	Detekce MALDI-TOF hmotnostní spektrometrií specifických spektrálních márkerů izolátů Coxiella burnetii (cs) Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry (en)
skos:prefLabel	Detekce MALDI-TOF hmotnostní spektrometrií specifických spektrálních márkerů izolátů Coxiella burnetii (cs) Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry (en)
skos:notation	RIV/60162694:G44__/07:00001856!RIV08-MO0-G44_____
http://linked.open.../vavai/riv/strany	55-58
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(OBVLAJEP20031)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Hernychová, Lenka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany - Fakulta vojenského zdravotnictví Hradec Králové
http://linked.open...dnocenehoVysledku	416548
http://linked.open...ai/riv/idVysledku	RIV/60162694:G44__/07:00001856
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bacteria; mass spectrometry; proteome; biomarkers (en)
http://linked.open.../riv/klicoveSlovo	bacteria biomarkers mass spectrometry proteome
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[1EB22704DD8D]
http://linked.open...i/riv/nazevZdroje	Acta Virologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	INFECTION 2 - The use of proteome analysis in microbiological diagnostics of infections whose aetiological agentsare on the listof BW-agents
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	51
http://linked.open...iv/tvurceVysledku	Hernychová, Lenka Skultety, L. Toman, Rudolf Bereghazyova, Eva Slaba, Katarina
issn	0001-723X
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	G44

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