About: Piezoelectric biosensor for a simple serological diagnosis of tularemia in infected European brown hares (Lepus europaeus)     Goto   Sponge   NotDistinct   Permalink

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Description
  • Piezoelectric biosensor was used for diagnosis of infection by Francisella tularensis subsp. holarctica in European brown hares. Two kinds of experiments were performed in this study. First, sera from experimentally infected European brown hares (Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day post infection was found as the first one when statistically significant diagnosis of tularemia was possible; all other sera collected from hares later than on day 7 following the infection were found tularemia positive. Typing to classify the field strain of F. tularensis used for the experimental infection was confirmed by proteome study. Second, sera from 35 European brown hare specimens sampled at hunting grounds and tested as tularemia positive by slow agglutination allowed diagnosis of tularemia by the piezoelectric biosensor. All these sera of naturally infected hares were found as tularemia positive, too. Efficacy of the piezoelectric biosensor for the serological diagnosis o
  • Piezoelectric biosensor was used for diagnosis of infection by Francisella tularensis subsp. holarctica in European brown hares. Two kinds of experiments were performed in this study. First, sera from experimentally infected European brown hares (Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day post infection was found as the first one when statistically significant diagnosis of tularemia was possible; all other sera collected from hares later than on day 7 following the infection were found tularemia positive. Typing to classify the field strain of F. tularensis used for the experimental infection was confirmed by proteome study. Second, sera from 35 European brown hare specimens sampled at hunting grounds and tested as tularemia positive by slow agglutination allowed diagnosis of tularemia by the piezoelectric biosensor. All these sera of naturally infected hares were found as tularemia positive, too. Efficacy of the piezoelectric biosensor for the serological diagnosis o (en)
  • Pro diagnózu infekce zajíců bakterií Francisella tularensis subsp. holarctica byl použit piezoelektrický biosensor. Dva experimentální postupy byly použity. Nejdříve byla séra experimentálně infikovaných zajíců stanovována piezoelektricky. 7 den po infekci byl prokázán jako první statisticky významný bod, kdy byla prokázána infekce. Všechna následující měření vakazoval pozitívní výsledek infekce. Typizace divokého izolátu kmene F. tularensis byla prokázána proteomickou studií. Druhý experimentální postup zahrnoval 35 zaječích vzorků posbíraných při honech a které byly positivní na tularemii pomocí metody pomalé aglutinace. Séra byla piezoelektricky testovánaa u všech byla prokázána přítomnost tularemie i peizoelektrickým biosensorem. Účinnost piezoelektrického biosenzoru pro serologické vyčetření je diskutována. (cs)
Title
  • Piezoelectric biosensor for a simple serological diagnosis of tularemia in infected European brown hares (Lepus europaeus)
  • Piezoelektrický senzor pro jednoduché serologické stanovení Tularémie v infikovaných zajících (Lepus euroaeus) (cs)
  • Piezoelectric biosensor for a simple serological diagnosis of tularemia in infected European brown hares (Lepus europaeus) (en)
skos:prefLabel
  • Piezoelectric biosensor for a simple serological diagnosis of tularemia in infected European brown hares (Lepus europaeus)
  • Piezoelektrický senzor pro jednoduché serologické stanovení Tularémie v infikovaných zajících (Lepus euroaeus) (cs)
  • Piezoelectric biosensor for a simple serological diagnosis of tularemia in infected European brown hares (Lepus europaeus) (en)
skos:notation
  • RIV/60162694:G44__/07:00001757!RIV08-MO0-G44_____
http://linked.open.../vavai/riv/strany
  • 2825-2834
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(OPUOFVZ200602), Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika
  • 0
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 441366
http://linked.open...ai/riv/idVysledku
  • RIV/60162694:G44__/07:00001757
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Francisella tularensis; zoonosis; reservoir host; experimental and natural infection; serology; immunosensor; agglutination test (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV
  • [6BCC77380D59]
http://linked.open...i/riv/nazevZdroje
  • Sensors
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 7
http://linked.open...iv/tvurceVysledku
  • Banďouchová, Hana
  • Beklová, Miroslava
  • Hubálek, Martin
  • Pikula, Jiří
  • Pohanka, Miroslav
  • Treml, František
http://linked.open...n/vavai/riv/zamer
issn
  • 1424-8220
number of pages
http://localhost/t...ganizacniJednotka
  • G44
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