About: Construction of a Francisella tularensis two-dimensional electrophoresis protein database

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight proteins were unambigu We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight proteins were unambigu (en) Konstrukce 2-DE proteinové databáze Francisella tularensis (cs)
Title	Construction of a Francisella tularensis two-dimensional electrophoresis protein database Construction of a Francisella tularensis two-dimensional electrophoresis protein database (en) Konstrukce 2-DE proteinové databáze Francisella tularensis (cs)
skos:prefLabel	Construction of a Francisella tularensis two-dimensional electrophoresis protein database Construction of a Francisella tularensis two-dimensional electrophoresis protein database (en) Konstrukce 2-DE proteinové databáze Francisella tularensis (cs)
skos:notation	RIV/60162694:G38__/01:00000499!RIV/2005/MO0/G38005/N
http://linked.open.../vavai/riv/strany	508-515
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LN00A033), P(MO66021299105), P(NI4700), Z(AV0Z5020903)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Malina, Michal Hernychová, Lenka Stulík, Jiří Macela, Aleš Kroča, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany (nerozlišená součást)
http://linked.open...dnocenehoVysledku	676304
http://linked.open...ai/riv/idVysledku	RIV/60162694:G38__/01:00000499
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Francisella tularensis;two-dimensional electrophoresis;protein database (en)
http://linked.open.../riv/klicoveSlovo	protein database two-dimensional electrophoresis Francisella tularensis
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[225857A9131C]
http://linked.open...i/riv/nazevZdroje	Proteomics
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	The proteome center for the study of intracellular parasitism of microbes Biological detection of microbial agens Francisella tularensis Local immune response of host against transdermal infection with intracellular bacterial pathogen Francisella tularensis
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	1
http://linked.open...iv/tvurceVysledku	Halada, Petr Hernychová, Lenka Stulík, Jiří Macela, Aleš Malina, Michal Kroča, Michal Johansson, Anders
http://linked.open...n/vavai/riv/zamer	Výzkum buněčné a molekulární biologie, genetiky, fyziologie a ekologie mikroorganizmů, mikrobní biotechnologie; studium imunitních procesů
issn	1615-9853
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	G38

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