About: Modulation of signal transduction pathways and global protein composition of macrophages by ionizing radiation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	It is assumed that the exposure of cells to ionizing radiation modulates theirsignal transduction pathways, which then govern the early and lateradiation-induced alterations in gene expression. In this study we tested theeffects of low doses of X-irradiation on the cell signaling and global proteincomposition of an HL-60 human promyelocytic leukemia cell line differentiatedalong a macrophage-like cell pathway by 4beta-phorbol-12-myristate-13-acetate(PMA). Using sodium dodecyl sulfate-polyacrylamide gelelectrophoresis(SDS-PAGE) followed by immunoblotting of anti-phosphotyrosineimmunoprecipitates, we found radiation-induced changes in the level ofphosphorylation of proteins with molecular masses of 45 and 48 kDa, but in themost intensively stained area,ranging from 54 to 60 kDa, no alterations wereobserved. When two-dimensional electrophoresis (2-DE) immunoblotting wasapplied, only proteins from this heavily stained region were visualized and inaddition the evident differences in tyrosine phosphorylat It is assumed that the exposure of cells to ionizing radiation modulates theirsignal transduction pathways, which then govern the early and lateradiation-induced alterations in gene expression. In this study we tested theeffects of low doses of X-irradiation on the cell signaling and global proteincomposition of an HL-60 human promyelocytic leukemia cell line differentiatedalong a macrophage-like cell pathway by 4beta-phorbol-12-myristate-13-acetate(PMA). Using sodium dodecyl sulfate-polyacrylamide gelelectrophoresis(SDS-PAGE) followed by immunoblotting of anti-phosphotyrosineimmunoprecipitates, we found radiation-induced changes in the level ofphosphorylation of proteins with molecular masses of 45 and 48 kDa, but in themost intensively stained area,ranging from 54 to 60 kDa, no alterations wereobserved. When two-dimensional electrophoresis (2-DE) immunoblotting wasapplied, only proteins from this heavily stained region were visualized and inaddition the evident differences in tyrosine phosphorylat (en) Modulace signálních cest a globálního proteinového složení makrofágů ionizujícím zářením (cs)
Title	Modulation of signal transduction pathways and global protein composition of macrophages by ionizing radiation Modulation of signal transduction pathways and global protein composition of macrophages by ionizing radiation (en) Modulace signálních cest a globálního proteinového složení makrofágů ionizujícím zářením (cs)
skos:prefLabel	Modulation of signal transduction pathways and global protein composition of macrophages by ionizing radiation Modulation of signal transduction pathways and global protein composition of macrophages by ionizing radiation (en) Modulace signálních cest a globálního proteinového složení makrofágů ionizujícím zářením (cs)
skos:notation	RIV/60162694:G38__/00:00000447!RIV/2005/MO0/G38005/N
http://linked.open.../vavai/riv/strany	382-388
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LN00A033), Z(MO0990200X003)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Koupilová, Kamila Hernychová, Lenka Stulík, Jiří Bláha, Václav Macela, Aleš
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany (nerozlišená součást)
http://linked.open...dnocenehoVysledku	717963
http://linked.open...ai/riv/idVysledku	RIV/60162694:G38__/00:00000447
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	genome;proteome (en)
http://linked.open.../riv/klicoveSlovo	genome proteome
http://linked.open...ontrolniKodProRIV	[1B5CAB9FE3AE]
http://linked.open...i/riv/mistoVydani	Weinheim
http://linked.open...vEdiceCisloSvazku	Neuveden
http://linked.open...i/riv/nazevZdroje	From genome to proteome
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	The proteome center for the study of intracellular parasitism of microbes
http://linked.open...UplatneniVysledku	2000
http://linked.open...iv/tvurceVysledku	Bláha, Václav Hernychová, Lenka Stulík, Jiří Macela, Aleš Baaske, Claudia Kaffenberger, Walter Koupilová, Kamila van Beuningen, Dirk
http://linked.open...n/vavai/riv/zamer	Prevence v hygieně a epidemiologii
number of pages	7 (xsd:int)
http://purl.org/ne...btex#hasPublisher	WILEY
https://schema.org/isbn	3-527-30154-2
http://localhost/t...ganizacniJednotka	G38

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