About: Toward the construction of Francisella tularensis 2-DE database

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Francisella tularensis is an obligate intracellular pathogen whose genome sequencing will be completed shortly. The next step should be the analysis of functional complement of genetic information so called proteome. Proteom is a new rapidly emerging field of study utilizing the combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry.We applied 2-DE methodology with immobilized pH gradients in the first dimension for separation of complete protein lysates of attenuated Francisella tularensis strain LVS. Two different techniques 9-16 % gradient SDS-PAGE and Tricine SDS-PAGE were used in the second dimension for the resolution of proteins in the range of molecular weights 10 to 200 kDa and 5-70 kDa, respectively. In the first case about 2700 proteins were detected on one gel, regarding Tricine SDS-PAGE we were able to stain approximately 1200 different protein molecules. Besides the cultivation of bacteria under normal condition we also examined the protein expression in heat-sh Francisella tularensis is an obligate intracellular pathogen whose genome sequencing will be completed shortly. The next step should be the analysis of functional complement of genetic information so called proteome. Proteom is a new rapidly emerging field of study utilizing the combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry.We applied 2-DE methodology with immobilized pH gradients in the first dimension for separation of complete protein lysates of attenuated Francisella tularensis strain LVS. Two different techniques 9-16 % gradient SDS-PAGE and Tricine SDS-PAGE were used in the second dimension for the resolution of proteins in the range of molecular weights 10 to 200 kDa and 5-70 kDa, respectively. In the first case about 2700 proteins were detected on one gel, regarding Tricine SDS-PAGE we were able to stain approximately 1200 different protein molecules. Besides the cultivation of bacteria under normal condition we also examined the protein expression in heat-sh (en) K vytvoření Francisella tularensis 2-DE datebáze (cs)
Title	Toward the construction of Francisella tularensis 2-DE database Toward the construction of Francisella tularensis 2-DE database (en) K vytvoření Francisella tularensis 2-DE datebáze (cs)
skos:prefLabel	Toward the construction of Francisella tularensis 2-DE database Toward the construction of Francisella tularensis 2-DE database (en) K vytvoření Francisella tularensis 2-DE datebáze (cs)
skos:notation	RIV/60162694:G38__/00:00000302!RIV/2005/MO0/G38005/N
http://linked.open.../vavai/riv/strany	29-30
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LN00A033), Z(MO0990200X003)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Hernychová, Lenka Stulík, Jiří Macela, Aleš Kroča, Michal
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ministerstvo obrany / Univerzita obrany (nerozlišená součást)
http://linked.open...dnocenehoVysledku	729431
http://linked.open...ai/riv/idVysledku	RIV/60162694:G38__/00:00000302
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	construction;Francisella tularensis;2-DE database (en)
http://linked.open.../riv/klicoveSlovo	construction Francisella tularensis 2-DE database
http://linked.open...ontrolniKodProRIV	[D18582160676]
http://linked.open...v/mistoKonaniAkce	Umea, Sweden
http://linked.open...i/riv/mistoVydani	Umea
http://linked.open...i/riv/nazevZdroje	The 3rd International Conference on Tularemia
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The proteome center for the study of intracellular parasitism of microbes
http://linked.open...UplatneniVysledku	2000
http://linked.open...iv/tvurceVysledku	Hernychová, Lenka Novák, Petr Stulík, Jiří Macela, Aleš Kroča, Michal
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2000-08-27 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Prevence v hygieně a epidemiologii
issn	1104-9154
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	FOA Defence Research Establishment
http://localhost/t...ganizacniJednotka	G38

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