About: Introduction to protoplast culture of Allium genus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Introduction to protoplast culture of Allium genus Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to establish the protoplast culture of Allium genus and verify the influence of electric field on regeneration ability of isolated protoplasts. Another goal was tested the possibilities of somatic hybridization in this genus. Mesophyll protoplasts were isolated from in vitro cultured plants of Allium flavum, A. fistulosum, A. schoenoprasum and A. ursinum. The protoplasts were isolated and fused using established protocol for Solanum genus: http://www.vubhb.cz/_te.asp?f=institute/06dogr-protocols-mfpep.htm with prolonged time of enzyme action. The induction of regeneration ability was tested using five parameters of electric field. The regeneration ability of affected protoplasts and fused protoplasts (A. flavum + A. ursinum; A. fistulosum + A. ursinum; A. fistulosum + A. schoenoprasum) was compared with non-affected control. All cultivated protoplasts maintained viability. The cell wall regeneration and cell division were observed in different time and intensity. The aim of this study was to establish the protoplast culture of Allium genus and verify the influence of electric field on regeneration ability of isolated protoplasts. Another goal was tested the possibilities of somatic hybridization in this genus. Mesophyll protoplasts were isolated from in vitro cultured plants of Allium flavum, A. fistulosum, A. schoenoprasum and A. ursinum. The protoplasts were isolated and fused using established protocol for Solanum genus: http://www.vubhb.cz/_te.asp?f=institute/06dogr-protocols-mfpep.htm with prolonged time of enzyme action. The induction of regeneration ability was tested using five parameters of electric field. The regeneration ability of affected protoplasts and fused protoplasts (A. flavum + A. ursinum; A. fistulosum + A. ursinum; A. fistulosum + A. schoenoprasum) was compared with non-affected control. All cultivated protoplasts maintained viability. The cell wall regeneration and cell division were observed in different time and intensity. (en)
Title	Introduction to protoplast culture of Allium genus Introduction to protoplast culture of Allium genus (en)
skos:prefLabel	Introduction to protoplast culture of Allium genus Introduction to protoplast culture of Allium genus (en)
skos:notation	RIV/60109807:_____/12:#0000224!RIV14-MSM-60109807
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(LF11012)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Domkářová, Jaroslava Polzerová, Hana Greplová, Marie
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...dnocenehoVysledku	142803
http://linked.open...ai/riv/idVysledku	RIV/60109807:_____/12:#0000224
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Allium flavum; A. fistulosum; A. schoenoprasum; A. ursinum; protoplast fusion (en)
http://linked.open.../riv/klicoveSlovo	Allium flavum protoplast fusion A. fistulosum A. schoenoprasum A. ursinum
http://linked.open...ontrolniKodProRIV	[B8F3DD3A3F2E]
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnological production process of Allium ursinum for food- and pharmaceutical industry.
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Domkářová, Jaroslava Greplová, Marie Polzerová, Hana

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software