About: Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

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About: Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET- 45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigens were used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus. The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET- 45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigens were used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus. (en)
Title	Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein (en)
skos:prefLabel	Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein (en)
skos:notation	RIV/60109807:_____/12:#0000202!RIV13-MSM-60109807
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	2012.01886
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Dědič, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...dnocenehoVysledku	162482
http://linked.open...ai/riv/idVysledku	RIV/60109807:_____/12:#0000202
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Potato virus M, recombinant protein, coat protein, triple gene block, polyclonal antibodies (en)
http://linked.open.../riv/klicoveSlovo	recombinant protein Potato virus M coat protein triple gene block polyclonal antibodies
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[65455F69913B]
http://linked.open...i/riv/nazevZdroje	J Phytopathol
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional genomics and proteomics for crop improvement
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	160
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Hoffmeisterová, Hana Plchová, Helena Čeřovská, Noemi Dědič, Petr
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1439-0434
number of pages	3 (xsd:int)

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