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rdf:type
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Description
| - Variability in forty somatic hybrids [S. bulbocastanum Dun. (2n=2x=24; cytoplasm type Wγ) + S. tuberosum (2n=2x=24; cytoplasm type Tßγ)] was assessed using in vitro morphology (general habitus, leaf shape and growth vigour), ploidy level, random amplified polymorphic DNA, microsatellites, mitochondrial DNA and chloroplast DNA analyses. Somatic hybrids with the sum of parental ploidy levels were not recorded in the expected percent. In contrast, hexaploid regenerants were more common. Some somatic hybrids were asymmetric. The influence of ploidy level and genome loss had no impact on in vitro morphology. Cytoplasm type influenced leaf shape only. Chloroplasts segregated in a random manner while cosegregation of chloroplasts was sporadic. However, the co-existence of parental types was recorded in all cases in the mitochondrial genome. Somatic hybrids with the cytoplasm type Tβγ showed mostly standard habitus close to in vitro S. tuberosum plants, whereas the hybrids with Wβγ cytoplasm showed branched or standard habitus. Variability among somatic hybrids can be successfully distinguished at the in vitro stage of the plant. This raises the possibility of preselection of prospective somatic hybrids for use in subsequent breeding programmes.
- Variability in forty somatic hybrids [S. bulbocastanum Dun. (2n=2x=24; cytoplasm type Wγ) + S. tuberosum (2n=2x=24; cytoplasm type Tßγ)] was assessed using in vitro morphology (general habitus, leaf shape and growth vigour), ploidy level, random amplified polymorphic DNA, microsatellites, mitochondrial DNA and chloroplast DNA analyses. Somatic hybrids with the sum of parental ploidy levels were not recorded in the expected percent. In contrast, hexaploid regenerants were more common. Some somatic hybrids were asymmetric. The influence of ploidy level and genome loss had no impact on in vitro morphology. Cytoplasm type influenced leaf shape only. Chloroplasts segregated in a random manner while cosegregation of chloroplasts was sporadic. However, the co-existence of parental types was recorded in all cases in the mitochondrial genome. Somatic hybrids with the cytoplasm type Tβγ showed mostly standard habitus close to in vitro S. tuberosum plants, whereas the hybrids with Wβγ cytoplasm showed branched or standard habitus. Variability among somatic hybrids can be successfully distinguished at the in vitro stage of the plant. This raises the possibility of preselection of prospective somatic hybrids for use in subsequent breeding programmes. (en)
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Title
| - Study of variability in population of somatic hybrids Solanum bulbocastanum (+) S. tuberosum
- Study of variability in population of somatic hybrids Solanum bulbocastanum (+) S. tuberosum (en)
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skos:prefLabel
| - Study of variability in population of somatic hybrids Solanum bulbocastanum (+) S. tuberosum
- Study of variability in population of somatic hybrids Solanum bulbocastanum (+) S. tuberosum (en)
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skos:notation
| - RIV/60109807:_____/12:#0000173!RIV13-MSM-60109807
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60109807:_____/12:#0000173
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - chloroplasts; cytoplasm-nuclear interaction; in vitro; mitochondria; somatic hybridization (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Vědecké práce – Výzkumný ústav bramborářský Havlíčkův Brod
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Domkářová, Jaroslava
- Greplová, Marie
- Polzerová, Hana
- Patzak, Josef
- Kreuz, Lukáš
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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