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  • 136 diploid genotypes of genus Solanum and diploid potato were selected and characterised as the potential resources of resistance genes against potato late blight. For a consideration of infectious tests by inoculation of leaf discs and in vitro plants, 15 highly resistant genotypes of wild species were selected; 6 of them showed no symptoms. In contrast, all 11 tested diploids of Solanum tuberosum were susceptible. Finally there were selected and characterised in detail 20 clones (10 clones of Solanum bulbocastanum, 1 clone of Solanum verrucosum and 9 clones of diploid Solanum tuberosum) that were useful for somatic hybridisation by electrofusion. The protoplast yield ranged between 1 x 105 and 3,5 x 106 per ml from on average 325 mg of plant leaf material and it was variable within single repetitions of one genotype too. The positive correlation between yield and vitality of isolated protoplasts was detected, r = 0.476 (mesoscale dependence).
  • 136 diploid genotypes of genus Solanum and diploid potato were selected and characterised as the potential resources of resistance genes against potato late blight. For a consideration of infectious tests by inoculation of leaf discs and in vitro plants, 15 highly resistant genotypes of wild species were selected; 6 of them showed no symptoms. In contrast, all 11 tested diploids of Solanum tuberosum were susceptible. Finally there were selected and characterised in detail 20 clones (10 clones of Solanum bulbocastanum, 1 clone of Solanum verrucosum and 9 clones of diploid Solanum tuberosum) that were useful for somatic hybridisation by electrofusion. The protoplast yield ranged between 1 x 105 and 3,5 x 106 per ml from on average 325 mg of plant leaf material and it was variable within single repetitions of one genotype too. The positive correlation between yield and vitality of isolated protoplasts was detected, r = 0.476 (mesoscale dependence). (en)
Title
  • CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS
  • CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS (en)
skos:prefLabel
  • CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS
  • CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS (en)
skos:notation
  • RIV/60109807:_____/09:0R000947!RIV10-MZE-60109807
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QF4107)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 306793
http://linked.open...ai/riv/idVysledku
  • RIV/60109807:_____/09:0R000947
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • potato; genetic resources; resistance against Phytophthora infestans; infectious tests; somatic hybridization; RAPD (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • SK - Slovenská republika
http://linked.open...ontrolniKodProRIV
  • [BAB3C08263C6]
http://linked.open...i/riv/nazevZdroje
  • Polnohospodarstvo
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 55
http://linked.open...iv/tvurceVysledku
  • Domkářová, Jaroslava
  • Sedlák, Petr
  • Vejl, Pavel
  • Sedláková, Vladimíra
  • Škodáček, Zbyněk
  • Horáčková, Vendulka
issn
  • 0551-3677
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