About: IMPROVED DETECTION OF PVS BY MOLECULAR HYBRIDIZATION AND BIOLISTIC INOCULATION

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About: IMPROVED DETECTION OF PVS BY MOLECULAR HYBRIDIZATION AND BIOLISTIC INOCULATION Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byla vyvinuta nová extrakční procedura pro detekci viru S a viru Y bramboru pomocí dot-blot hybridizace. Zatímco klasické AMESS extrakční postupy byly pro tyto viry neefektivní, citlivá detekce byla dosažena pomocí pufru s 5% SDS též bez nutnosti purifikovat RNA. Pro PVS byla rovněž vyvinuta alternativní, biolistická metoda inokulace. Touto metodou byl purifikovaným PVS dosaženo silného patogenního účinku u hostitelské rostlin rajčete cv. Něvské a rovněž byla prokázána vysoká úroveň akumulace tohoto viru v rostlině. Dot-blot hybridizace se sondami překlenující vybrané úseky génomu PVS (%22bypass sondy) citlivě detekovala infikované rostliny, bez nutnosti purifikovat celkovou RNA. (cs) New extraction procedure was developed to detect potato viruses S and Y by a dot-blot hybridization. While a classical AMESS extraction is ineffective for these potato viruses an improved extraction buffer that contains 5% SDS enabled a sensitive detection without need to purify RNA with an expensive procedures. An alternative biolistic inoculation method was developed for potato virus S (PVS). Biolistic inoculation with CsCl-purified PVS led to strong pathogenic effect on tomato cv. Nevske and to high levels of PVS accumulation. As detected by the dot-blot hybridization with a %22bypass%22 PVS probe, an infection was achieved even after biolistic inoculation with total RNA isolated from infected plants i.e. without any need to purify virus RNA. This suggests much simpler preparation of biolistic inocula for special handling of plant viruses and their mixtures. New extraction procedure was developed to detect potato viruses S and Y by a dot-blot hybridization. While a classical AMESS extraction is ineffective for these potato viruses an improved extraction buffer that contains 5% SDS enabled a sensitive detection without need to purify RNA with an expensive procedures. An alternative biolistic inoculation method was developed for potato virus S (PVS). Biolistic inoculation with CsCl-purified PVS led to strong pathogenic effect on tomato cv. Nevske and to high levels of PVS accumulation. As detected by the dot-blot hybridization with a %22bypass%22 PVS probe, an infection was achieved even after biolistic inoculation with total RNA isolated from infected plants i.e. without any need to purify virus RNA. This suggests much simpler preparation of biolistic inocula for special handling of plant viruses and their mixtures. (en)
Title	IMPROVED DETECTION OF PVS BY MOLECULAR HYBRIDIZATION AND BIOLISTIC INOCULATION ZLEPŚENÁ DETEKCE VIRU S BRAMBORU (PVS) MOLEKULÁRNÍ HYBRIDIZACÍ A BIOLOSTICKÁ INOKULACE. (cs) IMPROVED DETECTION OF PVS BY MOLECULAR HYBRIDIZATION AND BIOLISTIC INOCULATION (en)
skos:prefLabel	IMPROVED DETECTION OF PVS BY MOLECULAR HYBRIDIZATION AND BIOLISTIC INOCULATION ZLEPŚENÁ DETEKCE VIRU S BRAMBORU (PVS) MOLEKULÁRNÍ HYBRIDIZACÍ A BIOLOSTICKÁ INOKULACE. (cs) IMPROVED DETECTION OF PVS BY MOLECULAR HYBRIDIZATION AND BIOLISTIC INOCULATION (en)
skos:notation	RIV/60109807:_____/07:8P001891!RIV08-MZE-60109807
http://linked.open.../vavai/riv/strany	141-148
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QF3109)
http://linked.open...iv/cisloPeriodika	prosinec
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Dědič, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...dnocenehoVysledku	425762
http://linked.open...ai/riv/idVysledku	RIV/60109807:_____/07:8P001891
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Potato virus S; extraction; dot-blot; RNA (en)
http://linked.open.../riv/klicoveSlovo	RNA extraction dot-blot Potato virus S
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[BDC30E061585]
http://linked.open...i/riv/nazevZdroje	Vědecké práce
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The differentiation of Potato Virus S (PVS) and the identification of isolates of quarantine significance by means of molecular diagnostic techniques.
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Matoušek, J. Dědič, Petr
issn	1802-940X
number of pages	8 (xsd:int)

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