| Attributes | Values |
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| rdf:type
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| Description
| - Byly optimalizovány podmínky inaktivace organel. Úsilí bylo zaměřeno na přenos mimojaderné dědičnosti Solanum verrucosum a zvýšení variability u kulturního bramboru S.tuberosum. Protoplasty byly ozářeny UV světlem nebo ošetřeny JODOACETAMID (0.3 mM; 0.4 mM), RHODAMINE 6G (0.05 mM; 0.1 mM) a jodooctová kyselina(0.125 mM; 0.2 mM). Výsledkem bylo optimalizování koncentrace a doby působení. Ošetřené protoplasty byly fúzovány elektrickým polem nebo PEG. V asymetrických fúzích bylo dosaženo vyšších stupňů regenerace než u ošetřených kontrol. (cs)
- Optimalization of protoplast organelles inactivation (nuclei, chloroplasts, mitochondria) with goals using them in cybridization is aim of this study. The effort is focused especially on use extranuclei inheritance of wild species to increase variability of cultivated potato. Inactivation of nuclei and semiautonomic organelles was studied in mesophyll protoplasts selected from the members of the genus Solanum tuberosum (cv. Kordoba, cv. Karin, cv. Komtesa) and Solanum verrucosum. Immediately after the isolation, an aliquot of protoplasts was UV-irradiated, these protoplasts did not regenerate. Inactivation of semiautonomic organelles was performed in aliquots of protoplasts with graded concentrations of IODOACETAMIDE (0.3 mM; 0.4 mM), RHODAMINE 6G (0.05 mM; 0.1 mM) and IODOACETIC ACID (0.125 mM; 0.2 mM). These concentrations of active substances did not damage protoplasts and the regeneration of a cell wall was sporadically recorded. In controls, division was observed and microcolonies, microcalli
- Optimalization of protoplast organelles inactivation (nuclei, chloroplasts, mitochondria) with goals using them in cybridization is aim of this study. The effort is focused especially on use extranuclei inheritance of wild species to increase variability of cultivated potato. Inactivation of nuclei and semiautonomic organelles was studied in mesophyll protoplasts selected from the members of the genus Solanum tuberosum (cv. Kordoba, cv. Karin, cv. Komtesa) and Solanum verrucosum. Immediately after the isolation, an aliquot of protoplasts was UV-irradiated, these protoplasts did not regenerate. Inactivation of semiautonomic organelles was performed in aliquots of protoplasts with graded concentrations of IODOACETAMIDE (0.3 mM; 0.4 mM), RHODAMINE 6G (0.05 mM; 0.1 mM) and IODOACETIC ACID (0.125 mM; 0.2 mM). These concentrations of active substances did not damage protoplasts and the regeneration of a cell wall was sporadically recorded. In controls, division was observed and microcolonies, microcalli (en)
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| Title
| - A study of asymetric somatic hybridization in genus Solanum.
- A study of asymetric somatic hybridization in genus Solanum. (en)
- Studium asymetrické somatické hybridizace u rodu Solanum. (cs)
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| skos:prefLabel
| - A study of asymetric somatic hybridization in genus Solanum.
- A study of asymetric somatic hybridization in genus Solanum. (en)
- Studium asymetrické somatické hybridizace u rodu Solanum. (cs)
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| skos:notation
| - RIV/60109807:_____/07:8P001082!RIV08-MZE-60109807
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60109807:_____/07:8P001082
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - asymmetric protoplast fusion; Solanum; UV irradiation; IODOACETAMIDE; RHODAMINE 6G and IODOACETIC ACID (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Greplová, Marie
- Polzerová, Hana
|
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