About: Acclimatization of the somatic hybrids in the Solanum genus

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byla vybrána optimální metoda aklimatizace somatických hybridů rodu Solanum z in vitro do in vivo podmínek. (cs) Many valuable resistance genes are carried by wild species of Solanum genus that are often non-crossable with cultivated potato. Somatic hybridization is an approach overcoming this problem. The somatic hybrids were developed through protoplast fusion by an electric field (Navrátilová et al. 2006), they were evaluated by flow-cytometry, RAPD technique and their in vitro morphology. Optimal method for the most effective transfer into in vivo conditions was investigated. A set of somatic hybrids of two combinations was planted into seedling pots (3x3 cm) containing sterilized soil and cultured in a laboratory with artificial illumination (4000 Lux) of 16 h light/8 h dark photoperiod at 21 °C. The second variant involved direct planting into the pots (8x8 cm). Plants planted in this way were partially protected by non-woven fleece and exposed to weather conditions. Rate of survival and growth increment was investigated in both variants. Many valuable resistance genes are carried by wild species of Solanum genus that are often non-crossable with cultivated potato. Somatic hybridization is an approach overcoming this problem. The somatic hybrids were developed through protoplast fusion by an electric field (Navrátilová et al. 2006), they were evaluated by flow-cytometry, RAPD technique and their in vitro morphology. Optimal method for the most effective transfer into in vivo conditions was investigated. A set of somatic hybrids of two combinations was planted into seedling pots (3x3 cm) containing sterilized soil and cultured in a laboratory with artificial illumination (4000 Lux) of 16 h light/8 h dark photoperiod at 21 °C. The second variant involved direct planting into the pots (8x8 cm). Plants planted in this way were partially protected by non-woven fleece and exposed to weather conditions. Rate of survival and growth increment was investigated in both variants. (en)
Title	Acclimatization of the somatic hybrids in the Solanum genus Acclimatization of the somatic hybrids in the Solanum genus (en) Aklimatizace somatických hybridů rodu Solanum (cs)
skos:prefLabel	Acclimatization of the somatic hybrids in the Solanum genus Acclimatization of the somatic hybrids in the Solanum genus (en) Aklimatizace somatických hybridů rodu Solanum (cs)
skos:notation	RIV/60109807:_____/07:#0000073!RIV08-MSM-60109807
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(QF4108), V, Z(MSM6010980701)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Polzerová, Hana Greplová, Marie
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav bramborářský Havlíčkův Brod, s.r.o.
http://linked.open...dnocenehoVysledku	408375
http://linked.open...ai/riv/idVysledku	RIV/60109807:_____/07:#0000073
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	acclimatization; somatic hybrid; Solanum (en)
http://linked.open.../riv/klicoveSlovo	Solanum somatic hybrid acclimatization
http://linked.open...ontrolniKodProRIV	[E280681AA788]
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Utilization of protoplast fusion technique in breeding of the important cultivated crops of the genera Brassica, Cucumis and Solanum.
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Greplová, Marie Polzerová, Hana
http://linked.open...n/vavai/riv/zamer	Molekulární a technologické základy produkce kvalitních brambor

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