About: Lectin-binding characteristics of a Lyme borreliosis spirochete Borrelia burgdorferi sensu stricto

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About: Lectin-binding characteristics of a Lyme borreliosis spirochete Borrelia burgdorferi sensu stricto Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Borrelial glycoconjugates were localized by labeled lectins on ultrathin cryosections and on surfaces of intact negatively stained bacteria. We partially characterized protein-carbohydrate complexes in borrelial glycoconjugates by means of enzyme deglycosylation and mild alkali pretreatment of cryosections. Results of labeling were examined by transmission electron microscopy (TEM). Statistically evaluated results (relative labeling index, chi-squared test) of gold labeling has indicated that surfaces of Borrelia burgdorferi B31 and external (outer) membrane vesicles (MVs), were covered with glycoconjugates containing O-glycosidically linked N-acetyl galactosamine (GalNAc) and N-glycosidically linked N-acetyl-D-glucosamine (GlcNAc). Borrelial glycoconjugates were localized by labeled lectins on ultrathin cryosections and on surfaces of intact negatively stained bacteria. We partially characterized protein-carbohydrate complexes in borrelial glycoconjugates by means of enzyme deglycosylation and mild alkali pretreatment of cryosections. Results of labeling were examined by transmission electron microscopy (TEM). Statistically evaluated results (relative labeling index, chi-squared test) of gold labeling has indicated that surfaces of Borrelia burgdorferi B31 and external (outer) membrane vesicles (MVs), were covered with glycoconjugates containing O-glycosidically linked N-acetyl galactosamine (GalNAc) and N-glycosidically linked N-acetyl-D-glucosamine (GlcNAc). (en) Glykokonjugáty borrelií byly lokalizovány pomocí vazby lektinů na ultratenkých kryořezech a na povrchy negativně barvených bakterií. Protein-sacharidové komplexy jsme charakterizovali pomocí enzymatické glykosylace a alkalickým působením. Výsledky značení jsme sledovali v transmisním elektronovém mikroskopu. Statistické vyhodnocení výsledků ukázalo, že povrchy Borrelia burgdorferi B31 a vnější membránové váčky jsou pokryty glykokonjugáty, které obsahují O-vázaný N-acetyl-D-galactosamin a N-vázaný N-acetyl-D-glucosamin. (cs)
Title	Lectin-binding characteristics of a Lyme borreliosis spirochete Borrelia burgdorferi sensu stricto Lectin-binding characteristics of a Lyme borreliosis spirochete Borrelia burgdorferi sensu stricto (en) Lektinová charakterizace spirochéty Lymské boreliózy, Borrelia burgdorferi sensu stricto (cs)
skos:prefLabel	Lectin-binding characteristics of a Lyme borreliosis spirochete Borrelia burgdorferi sensu stricto Lectin-binding characteristics of a Lyme borreliosis spirochete Borrelia burgdorferi sensu stricto (en) Lektinová charakterizace spirochéty Lymské boreliózy, Borrelia burgdorferi sensu stricto (cs)
skos:notation	RIV/60077409:_____/05:00025636!RIV06-GA0-60077409
http://linked.open.../vavai/riv/strany	229;238
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/03/1323), P(KSK6005114), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Grubhoffer, Libor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Parazitologický ústav AV ČR
http://linked.open...dnocenehoVysledku	527919
http://linked.open...ai/riv/idVysledku	RIV/60077409:_____/05:00025636
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Borrelia burgdorferi; electron microscopy; lectin binding (en)
http://linked.open.../riv/klicoveSlovo	electron microscopy Borrelia burgdorferi lectin binding
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[963495A640E4]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biodiversity and functions of ecological systems Lyme borreliosis spirochetes: molecular factors of protein-carbohydrate interactions
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Grubhoffer, Libor Nebesářová, J. Vancová, M.
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	0015-5632
number of pages	10 (xsd:int)

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