About: Improved metod of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification

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About: Improved metod of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A simple assay by polymerace chain reaction was used for the detection of Borrelia burgdorferi, causative agent of Lyme borreliosis (LB). It involves no DNA purification and is based on the amplification of a specific region of ospA gene of B. burgdorferi, followed by direct detection of the PCR product with SYBR Green I by agarose gel electrophoresis. The proposed reliable, rapid, unexpensive and specific technique could form the basis of laboratory tests for routine and identification of Lyme-disease spirochete in different samples. A simple assay by polymerace chain reaction was used for the detection of Borrelia burgdorferi, causative agent of Lyme borreliosis (LB). It involves no DNA purification and is based on the amplification of a specific region of ospA gene of B. burgdorferi, followed by direct detection of the PCR product with SYBR Green I by agarose gel electrophoresis. The proposed reliable, rapid, unexpensive and specific technique could form the basis of laboratory tests for routine and identification of Lyme-disease spirochete in different samples. (en) Jednoduchá metoda s využitím polymerázové řetězové reakce byla využita k průkazu Borrelia burgdorferi, původce lymské boreliózy (LB). Nevyžaduje purifikaci DNA a je založena na amplifikaci specifické oblasti genu ospA B. burgdorferi s přímou detekcí produktu PCR pomocí SYBR Gree I na agarózové elektroforéze. Předložená metoda je spolehlivá, rychlá, levná a specifická technika, která by mohla představovat základ rutinní identifikace spirochét LB v různých typech vzorků. (cs)
Title	Improved metod of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification Improved metod of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification (en) Vylepšení průkazu a molekulární typizace Borrelia burgdorferi s.l. v klinických vzorcích metodou PCR bez purifikace DNA (cs)
skos:prefLabel	Improved metod of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification Improved metod of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification (en) Vylepšení průkazu a molekulární typizace Borrelia burgdorferi s.l. v klinických vzorcích metodou PCR bez purifikace DNA (cs)
skos:notation	RIV/60077409:_____/05:00025560!RIV06-GA0-60077409
http://linked.open.../vavai/riv/strany	31;39
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA524/03/1326), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Grubhoffer, Libor Rudenko, Natalia Golovchenko, Maryna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Parazitologický ústav AV ČR
http://linked.open...dnocenehoVysledku	524403
http://linked.open...ai/riv/idVysledku	RIV/60077409:_____/05:00025560
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR; chelex; amplification (en)
http://linked.open.../riv/klicoveSlovo	PCR amplification chelex
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[0634D8BAD9A6]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular typing and genetic characterization of Lyme borreliosis spirochetes.
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Golovchenko, Maryna Grubhoffer, Libor Mallátová, N. Rudenko, Natalia Němec, J. Volkaert, J.
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	0015-5632
number of pages	9 (xsd:int)

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