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| rdf:type
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| Description
| - Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the Leishmania donovani complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains that belong to nine zymodemes. Strains belonging to the species L. major and L. tropica were used as outgroups. A total of 902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% amplicons were shared by all species, while 4.3% amplicons were common for all 15 strains of the L. donovani complex. Well-supported trees have been constructed, which show a rather strong correlation between the genetic polymorphism of studied strains and their geographic origin. In all obtained trees, L. infantum was paraphyletic. The RAPD profiles suggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and other leishmanias does not warrant existence of a separate species
- Random amplified polymorphic DNA (RAPD) was used to detect intraspecific diversity for the Leishmania donovani complex. Fifty-two decameric to 21-meric primers of arbitrary sequence were applied to 15 strains that belong to nine zymodemes. Strains belonging to the species L. major and L. tropica were used as outgroups. A total of 902 amplicons generated by RAPD were scored. Most primers produced species-specific profiles, only 0.6% amplicons were shared by all species, while 4.3% amplicons were common for all 15 strains of the L. donovani complex. Well-supported trees have been constructed, which show a rather strong correlation between the genetic polymorphism of studied strains and their geographic origin. In all obtained trees, L. infantum was paraphyletic. The RAPD profiles suggest that MON-30 belongs to L. donovani. Moreover, the genetic distance between the L. archibaldi strain and other leishmanias does not warrant existence of a separate species (en)
- Polymorfismus náhodných sekvencích (RAPD) byl použit k detekci intraspecifické diverzity u L. donovani komplexu. Bylo použito 52 primerů (deseti až jednadvaceti merů), které amplifikovaly náhodné sekvence u 15 kmenů příslušícím k devíti zymodemovým skupinám. Jako outgroup byly použity L. major a L. tropica. Bylo generováno 902 amplikonů, z nichž pouhých 4.3% bylo shodných pro všech 15 kmenů L. donovani komplexu. Byl zkonstruován dobře podpořený fylogenetický strom, který podpořil silnou korelaci mezi genetickým polymorfismem a geografickým původem. L. infantum byla parafyletická u všech použitých algoritmů. MON-30 patří do L. donovani na základě RAPD profilu. A navíc, genetická vzdálenost kmene L. archibaldi a dalších leishmanií nepoukazuje na existencí jednotlivých druhů (cs)
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| Title
| - Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin
- Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin (en)
- Genetický polymorfismus uvnitř Leishmania donovani komplexu koreluje s jejich geografickým původem (cs)
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| skos:prefLabel
| - Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin
- Genetic polymorphism within the Leishmania donovani complex: correlation with geographic origin (en)
- Genetický polymorfismus uvnitř Leishmania donovani komplexu koreluje s jejich geografickým původem (cs)
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| skos:notation
| - RIV/60077409:_____/04:00104486!RIV/2005/AV0/A59005/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077409:_____/04:00104486
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - genetic polymorphism;Leishmania donovani;RAPD (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - American Journal of Tropical Medicine and Hygiene
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Jirků, Milan
- Lukeš, Julius
- Zemanová, Eva
- Mauricio, I. L.
- Miles, M. A.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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