About: Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum.

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About: Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to Neospora caninum, an important parasite of cattle and dogs. Oocysts of these two species are morphologically indistinguishable from each other. A population of 2240 dogs in the Czech Republic was screened for the presence of H. heydorni/N. caninum oocysts and five (0.22), represented by five of 3135 faecal samples (0.16), were positive. The internal transcribed spacer 1 region of the rRNA gene (ITS1) from two isolates were cloned and the DNA sequences were identical with those of the ITS1 of H. heydorni. Based on the rRNA sequences available for H. heydorni and related coccidia, the primer pair JS4-JS5 was designed to amplify the 3' end of the small subunit (SSU) rRNA gene and ITS1 of H. heydorni. When tested on DNA extracted from a variety of parasites, the primers amplified a specific 267 bp fragment in our isolates only. The presence of DNA equivalent to 10 oocysts was sufficient for the ampli Hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to Neospora caninum, an important parasite of cattle and dogs. Oocysts of these two species are morphologically indistinguishable from each other. A population of 2240 dogs in the Czech Republic was screened for the presence of H. heydorni/N. caninum oocysts and five (0.22), represented by five of 3135 faecal samples (0.16), were positive. The internal transcribed spacer 1 region of the rRNA gene (ITS1) from two isolates were cloned and the DNA sequences were identical with those of the ITS1 of H. heydorni. Based on the rRNA sequences available for H. heydorni and related coccidia, the primer pair JS4-JS5 was designed to amplify the 3' end of the small subunit (SSU) rRNA gene and ITS1 of H. heydorni. When tested on DNA extracted from a variety of parasites, the primers amplified a specific 267 bp fragment in our isolates only. The presence of DNA equivalent to 10 oocysts was sufficient for the ampli (en)
Title	Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum. Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum. (en)
skos:prefLabel	Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum. Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum. (en)
skos:notation	RIV/60077409:_____/02:59023031!RIV/2003/AV0/A59003/N
http://linked.open.../vavai/riv/strany	147;154
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA6022903), Z(AV0Z6022909)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Šlapeta, Jan Roger Modrý, David Koudela, Břetislav Lukeš, Julius
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Parazitologický ústav AV ČR
http://linked.open...dnocenehoVysledku	641782
http://linked.open...ai/riv/idVysledku	RIV/60077409:_____/02:59023031
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Hammondia heydorni; Neospora caninum; coprodiagnosis (en)
http://linked.open.../riv/klicoveSlovo	Hammondia heydorni Neospora caninum coprodiagnosis
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[81CF532F0C4D]
http://linked.open...i/riv/nazevZdroje	Veterinary Journal
http://linked.open...in/vavai/riv/obor	EG
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Phylogeny of bodonid and coccidian protozoa based on small subunit rRNA and conserved protein sequences
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	163
http://linked.open...iv/tvurceVysledku	Lukeš, Julius Modrý, David Koudela, Břetislav Votýpka, Jan Hořejš, R. Šlapeta, Jan Roger
http://linked.open...n/vavai/riv/zamer	Parazitické a symbiotické organismy živočichů a člověka: jejich vzájemné vztahy na úrovni populací, organismů, buněk a molekul
issn	1090-0233
number of pages	8 (xsd:int)

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