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Description
  • Dosud se předpokládalo, že většina extracelulárních enzymů je bakteriálního původu. Testovali jsme tento předpoklad na vztahu mezi bakteriálními počty a 3 glykolytickými enzymy ze 17 nádrží. Velký soubor dat tento předpoklad nepotvrdil, neboť počty baterií jen slabě korelovaly s aktivitou alfa- a beta-glukosidázy i beta-N-acetylhexosaminidázy. Maxima biomasy rozsivek těsně koreloval s aktivitou hexosaminidázy. Dále jsme prokázali těsnou korelaci mezi aktivitou obou glukosidáz a sušinou Daphnia longispina v alpském jezeře. Tato i jiné studie ukazují, že extracelulární glykolytické aktivity nelze jednoduše spojovat s bakteriemi. Také bezobratlí živočichové a další eukaryota (houby, rozsivky, prvoci, aj.) je nutno považovat za potenciálnš významné producenty enzymů. (cs)
  • It has been considered that most extracellular enzymes are of bacterial origin. We tested this paradigm by evaluating the relationship between bacterial number and the activity of three glycolytic enzymes from 17 fresh waters. Our large sets of pooled data do not seem to support such a simple explanation, because we found only a weak correlation of bacterial number with activity of alpha- and beta-glucosidases, and beta-N-acetylhexosaminidase. During diatom blooms, their biomass tightly correlated with hexosaminidase activity. We also documented very tight relationships between activity of both glucosidases and dry weight of Daphnia longispina in an alpine clear-water lake. Our data and evidence from other studies indicate that extracellular glycosidic activities in aquatic ecosystems cannot generally be assigned only to bacteria. Also invertebrate animals and other eukaryotes (fungi, diatoms, protozoa etc.) should be considered as potentially very important enzyme producers.
  • It has been considered that most extracellular enzymes are of bacterial origin. We tested this paradigm by evaluating the relationship between bacterial number and the activity of three glycolytic enzymes from 17 fresh waters. Our large sets of pooled data do not seem to support such a simple explanation, because we found only a weak correlation of bacterial number with activity of alpha- and beta-glucosidases, and beta-N-acetylhexosaminidase. During diatom blooms, their biomass tightly correlated with hexosaminidase activity. We also documented very tight relationships between activity of both glucosidases and dry weight of Daphnia longispina in an alpine clear-water lake. Our data and evidence from other studies indicate that extracellular glycosidic activities in aquatic ecosystems cannot generally be assigned only to bacteria. Also invertebrate animals and other eukaryotes (fungi, diatoms, protozoa etc.) should be considered as potentially very important enzyme producers. (en)
Title
  • Are bacteria the major producers of extracellular glycolytic enzymes in aquatic environments?
  • Jsou bakterie hlavními producenty extracelulárních glykolytických enzymů ve vodním prostředí? (cs)
  • Are bacteria the major producers of extracellular glycolytic enzymes in aquatic environments? (en)
skos:prefLabel
  • Are bacteria the major producers of extracellular glycolytic enzymes in aquatic environments?
  • Jsou bakterie hlavními producenty extracelulárních glykolytických enzymů ve vodním prostředí? (cs)
  • Are bacteria the major producers of extracellular glycolytic enzymes in aquatic environments? (en)
skos:notation
  • RIV/60077387:_____/04:00104268!RIV/2005/AV0/A62005/N
http://linked.open.../vavai/riv/strany
  • 102;117
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/00/0063), P(GA206/99/0028), P(IBS6017004), Z(AV0Z6017912)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 555344
http://linked.open...ai/riv/idVysledku
  • RIV/60077387:_____/04:00104268
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ectoenzyme activity; diatoms; Daphnia longispina (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [3083BE7E483A]
http://linked.open...i/riv/nazevZdroje
  • International Review of Hydrobiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 89
http://linked.open...iv/tvurceVysledku
  • Hejzlar, Josef
  • Šimek, Karel
  • Nedoma, Jiří
  • Vrba, Jaroslav
  • Macek, Miroslav
  • Hartman, Petr
  • Filandr, P.
  • Bittl, T.
  • Callieri, C.
  • Bertoni, R.
http://linked.open...n/vavai/riv/zamer
issn
  • 1434-2944
number of pages
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