About: Impact of ionic aluminium on extracellular phosphatases in acidified lakes.     Goto   Sponge   NotDistinct   Permalink

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  • Direct inhibiting effects of Al on extracellular phosphatases produced by the plankton were studied in lab experiments. Significant reduction of an apparent substrate affinity was found at Al concentrations 300-1000 g l<sup>-1</sup> at pH 4.5 and 4.8, while maximum acid phosphatase activity (APA) remained unchanged. Such behaviour of saturation kinetics is compatible with the assumption that ionic Al acts as a competitive inhibitor. To decide whether the observed Al effects could be explained alternatively by complexation of Al with substrate, the best fits of data with both possible models was statistically tested. Experimental results supported the competitive hypothesis rather than the complexation model. For pH values <5.2, the results of an Al-pH matrix experiment gave a more detailed picture: the higher the Al concentration, the wider was the pH range where Al could negatively affect APA. The ecological ramifications of this effect were evaluated in the context of field APA data on three strong
  • Direct inhibiting effects of Al on extracellular phosphatases produced by the plankton were studied in lab experiments. Significant reduction of an apparent substrate affinity was found at Al concentrations 300-1000 g l<sup>-1</sup> at pH 4.5 and 4.8, while maximum acid phosphatase activity (APA) remained unchanged. Such behaviour of saturation kinetics is compatible with the assumption that ionic Al acts as a competitive inhibitor. To decide whether the observed Al effects could be explained alternatively by complexation of Al with substrate, the best fits of data with both possible models was statistically tested. Experimental results supported the competitive hypothesis rather than the complexation model. For pH values <5.2, the results of an Al-pH matrix experiment gave a more detailed picture: the higher the Al concentration, the wider was the pH range where Al could negatively affect APA. The ecological ramifications of this effect were evaluated in the context of field APA data on three strong (en)
Title
  • Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
  • Impact of ionic aluminium on extracellular phosphatases in acidified lakes. (en)
skos:prefLabel
  • Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
  • Impact of ionic aluminium on extracellular phosphatases in acidified lakes. (en)
skos:notation
  • RIV/60077387:_____/01:62013084!RIV/2003/AV0/A62003/N
http://linked.open.../vavai/riv/strany
  • 578;587
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/00/0063), P(GA206/97/0072), Z(AV0Z6017912)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 682452
http://linked.open...ai/riv/idVysledku
  • RIV/60077387:_____/01:62013084
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • acid phosphatases; pH effect; inhibition (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [CF4DE69EEB10]
http://linked.open...i/riv/nazevZdroje
  • Environmental Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 3
http://linked.open...iv/tvurceVysledku
  • Kopáček, Jiří
  • Nedoma, Jiří
  • Vrba, Jaroslav
  • Bittl, T.
http://linked.open...n/vavai/riv/zamer
issn
  • 1462-2912
number of pages
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