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Description
  • V příspěvku jsou prezentovány základní principy kryoprezervačních metod, a to jak konzervativních tak vitrifikačních technik, a shrnuty faktory, které mohou ovlivnit úspěšnost kryoprezervace řas a sinic. Jedná se o faktory související s vlastním procesem kryoprezervace, které jsou specifické pro jednotlivé techniky i o faktory související s přípravou a manipulací vzorků před a po kryoprezervaci. Pozornost je věnována zejména vlivu kultivačních podmínek, především teplotě, v souvislosti s možností aplikace chladové aklimace při kryoprezervaci půdních řas. Jak z literárních údajů tak z naší zkušenosti vyplývá, že odezva půdních řas a sinic na jednotlivé faktory je druhově či kmenově specifická a že jejich optimalizace může vést k úspěšné kryoprezervaci řady dosud nezmrazitelných kmenů. (cs)
  • Fundamental principles of both conservative and vitrification cryopreservation methods are presented, and factors affecting cryopreservation of algae and cyanobacteria are summarized. These factors include factors connected with the cryopreservation process itself as well as factors connected with pre- and post-cryopreservation manipulation with samples. Attention is paid to cultivation conditions, mainly to the temperature in the context of application of cold acclimation before cryopreservation. Both literature data and our experience indicate that the response of soil algae and cyanobacteria to particular factors is species or strain specific and optimization of condition can enhance post-cryopreservation viability of cryopreservation recalcitrant strain.
  • Fundamental principles of both conservative and vitrification cryopreservation methods are presented, and factors affecting cryopreservation of algae and cyanobacteria are summarized. These factors include factors connected with the cryopreservation process itself as well as factors connected with pre- and post-cryopreservation manipulation with samples. Attention is paid to cultivation conditions, mainly to the temperature in the context of application of cold acclimation before cryopreservation. Both literature data and our experience indicate that the response of soil algae and cyanobacteria to particular factors is species or strain specific and optimization of condition can enhance post-cryopreservation viability of cryopreservation recalcitrant strain. (en)
Title
  • The effect of cultivation conditions on cryopreservation of soil algae and cyanobacteria
  • The effect of cultivation conditions on cryopreservation of soil algae and cyanobacteria (en)
  • Vliv kultivačních podmínek na kryoprezervaci půdních řas a sinic (cs)
skos:prefLabel
  • The effect of cultivation conditions on cryopreservation of soil algae and cyanobacteria
  • The effect of cultivation conditions on cryopreservation of soil algae and cyanobacteria (en)
  • Vliv kultivačních podmínek na kryoprezervaci půdních řas a sinic (cs)
skos:notation
  • RIV/60077379:_____/05:00030518!RIV06-AV0-60077379
http://linked.open.../vavai/riv/strany
  • 121;128
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z60660521)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 519269
http://linked.open...ai/riv/idVysledku
  • RIV/60077379:_____/05:00030518
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cultivation conditions; cryopreservation; soil algae (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [C33953208531]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Praha
http://linked.open...i/riv/nazevZdroje
  • Život v pode 6. Sborník z mezinárodního semináře
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Lukešová, Alena
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Česká zemědělská univerzita v Praze
https://schema.org/isbn
  • 80-213-1348-X
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