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| Description
| - Byly studovány cytotoxické účinky deseti metanolových extraktů půdních sinic (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix, Trichormus). Testování cytotoxicity bylo prováděno na 2 savčích buněčných liniích pomocí MTT testu a průtokové cytometrie (FC). Pro srovnání byl použit i test s Artemia jako testovacím organismem. Složení extraktů bylo studováno na HPLC-MS. U 6 kmenů z deseti testovaných byla nalezena cytotoxicita pomocí MTT a průtoková cytometrie, ale zjištění nekorelovala s testem pomocí Artemia. Největší poškození bylo zaznamenáno u Anabaena torulosa, Cylindrospermum sp. U buněk ošetřených extraktem Anabaena torulosa byla zjištěna 100% inhibice už po 60 min. expozici. Žádné účinky nebyly překvapivě zaznamenány na Artemia. U Cylindrospermum sp. byla pomocí FC zjištěna 80% nekrotická a 20% apoptická indukce. (cs)
- The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix, Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of 10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa,Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of A. torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane.
- The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix, Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of 10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa,Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of A. torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane. (en)
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| Title
| - Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI
- Cytotoxický efekt extraktů půdních sinic k liniím savčích buněk YAC-1 A WEHI (cs)
- Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI (en)
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| skos:prefLabel
| - Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI
- Cytotoxický efekt extraktů půdních sinic k liniím savčích buněk YAC-1 A WEHI (cs)
- Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI (en)
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| skos:notation
| - RIV/60077379:_____/05:00029096!RIV06-AV0-60077379
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077379:_____/05:00029096
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - cytotoxic effect; soil cyanobacterial extracts; mammal cell lines YAC-1 and WEHI (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hrouzek, Pavel
- Kopecký, Jiří
- Lukešová, Alena
- Maršálek, Blahoslav
- Salát, Jiří
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| http://linked.open...n/vavai/riv/zamer
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