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Description
  • Microbial communities of 4 different stages (initial [0 years after heaping of the excavated material], early [10], middle [20] and late [42] of primary succession process were studied. They were characterized by genotyping methods: ARDRA - Amplified ribosomal DNA restriction analyses and box-PCR - genomic fingerprinting using specific primer for boxA-element. Box-PCR method was chosen for comparison of methodological approaches used for characterization of heterotrophic bacterial communities. ARDRA patterns of eubacterial communities were so complex and there weren´t shown any differences among individual communities on different stages of primary succession process. Actinomycetes communities were analyzed using two sets of primers: Actinomycetes 1. (243F-1378R, Heuer et al. 1997) and Actinomycetes 2. (243F-A3R; Monciardini et al. 2002). Fingerprints, which were obtained using both of actinomycetes specific primers, showed differences between communities on the early and later stag...
  • Microbial communities of 4 different stages (initial [0 years after heaping of the excavated material], early [10], middle [20] and late [42] of primary succession process were studied. They were characterized by genotyping methods: ARDRA - Amplified ribosomal DNA restriction analyses and box-PCR - genomic fingerprinting using specific primer for boxA-element. Box-PCR method was chosen for comparison of methodological approaches used for characterization of heterotrophic bacterial communities. ARDRA patterns of eubacterial communities were so complex and there weren´t shown any differences among individual communities on different stages of primary succession process. Actinomycetes communities were analyzed using two sets of primers: Actinomycetes 1. (243F-1378R, Heuer et al. 1997) and Actinomycetes 2. (243F-A3R; Monciardini et al. 2002). Fingerprints, which were obtained using both of actinomycetes specific primers, showed differences between communities on the early and later stag... (en)
  • Byla studována mikrobiální společenstva 4 rozdílných stadií (iniciální [0 let po navršení výsypkového materiálu], rané [10], střední [20] a pokročilé [42]) v procesu primární sukcese. Tato společenstva byla charakterizována genotypickými metodami: ARDRA (Amplified Ribosomal DNA Restriction Analysis) a box-PCR - genomický fingerprint s použitím specifického primeru pro repetitivní box A - element. Metoda box-PCR byla vybrána pro porovnání metodických přístupů analýzy heterotrofního bakteriálního společenstva. ARDRA profily eubakteriálního společenstva byly příliš komplexní a neukázaly žádné rozdíly mezi jednotlivými společenstvy eubakterií na odlišných sukcesních plochách. Společenstvo aktinomycet bylo studováno použitím dvou sad primerů: aktinomycety 1. (243F-1378R, Heuer et al. 1997) a aktinomycety 2. (243F-1401R, Monciardini et al. 2002).Fingerprinty získané použitím dvou specifických sad primerů pro aktinomycety ukázaly rozdíl mezi společenstvem aktinomycet na raném a středním st... (cs)
Title
  • Analysis of bacterial isolates and community DNA from four different succession plots in post-mining area
  • Analysis of bacterial isolates and community DNA from four different succession plots in post-mining area (en)
  • Analýza bakteriálních izolátů a DNA ze společenstva ze čtyř různých sukcesních ploch na území po těžbě hnědého uhlí (cs)
skos:prefLabel
  • Analysis of bacterial isolates and community DNA from four different succession plots in post-mining area
  • Analysis of bacterial isolates and community DNA from four different succession plots in post-mining area (en)
  • Analýza bakteriálních izolátů a DNA ze společenstva ze čtyř různých sukcesních ploch na území po těžbě hnědého uhlí (cs)
skos:notation
  • RIV/60077379:_____/04:00105402!RIV/2005/GA0/A61005/N
http://linked.open.../vavai/riv/strany
  • 93;96
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA526/03/1259), Z(AV0Z6066911)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 554180
http://linked.open...ai/riv/idVysledku
  • RIV/60077379:_____/04:00105402
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • bacterial isolates;community DNA;succession plots in post-mining area (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [F2D09578EC6D]
http://linked.open...v/mistoKonaniAkce
  • České Budějovice
http://linked.open...i/riv/mistoVydani
  • České Budějovice
http://linked.open...i/riv/nazevZdroje
  • Present methods for investigation of microbial community biodiversity in soils and substrates
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Halbritter, A.
  • Krištůfek, Václav
  • Chroňáková, Alica
  • Biró, B.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Ústav půdní biologie AV ČR
https://schema.org/isbn
  • 80-86525-03-1
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