About: Phytoplasma infections in Rhododendron hybridum

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The survey of the health status of azaleas and rhododendrons on production areas in the Czech Republic started in 2006. Symptoms of unknown aetiology on Rhododendron hybridum cultivar Cunningham’s White were observed in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma-specific, universal and group specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma in the aster yellows subgroup 16SrI-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma from the infected rhododendron plant (1,724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. The survey of the health status of azaleas and rhododendrons on production areas in the Czech Republic started in 2006. Symptoms of unknown aetiology on Rhododendron hybridum cultivar Cunningham’s White were observed in 2010. The infected plant had malformed leaves, with irregular shaped edges, mosaic, leaf tip necrosis and multiple axillary shoots with smaller leaves. Transmission electron microscopy showed phytoplasma-like bodies in phloem cells of the symptomatic plant. Phytoplasma presence was confirmed by polymerase chain reaction using phytoplasma-specific, universal and group specific primer pairs. Restriction fragment length polymorphism analysis of 16S rDNA enabled classification of the detected phytoplasma in the aster yellows subgroup 16SrI-C. Sequence analysis of the 16S-23S ribosomal operon of the amplified phytoplasma from the infected rhododendron plant (1,724 bp) confirmed the closest relationship with the Czech Echinacea purpurea phyllody phytoplasma. (en)
Title	Phytoplasma infections in Rhododendron hybridum Phytoplasma infections in Rhododendron hybridum (en)
skos:prefLabel	Phytoplasma infections in Rhododendron hybridum Phytoplasma infections in Rhododendron hybridum (en)
skos:notation	RIV/60077344:_____/14:00439207!RIV15-AV0-60077344
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LD12074)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Fránová, Jana Přibylová, Jaroslava Špak, Josef
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	36613
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/14:00439207
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Electron microscopy; PCR; phytoplasma; sequencing (en)
http://linked.open.../riv/klicoveSlovo	Electron microscopy phytoplasma PCR sequencing
http://linked.open...ontrolniKodProRIV	[2B4401A8FA44]
http://linked.open...i/riv/mistoVydani	Bologna
http://linked.open...i/riv/nazevZdroje	Phytoplasmas and phytoplasma disease management: how to reduce their economic impact
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	287 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of phytoplasma genomic components and development of a microarray for their determination and management.
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Přibylová, Jaroslava Špak, Josef Fránová, Jana
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	IPWG - International Phytoplasmologist Working Group
https://schema.org/isbn	978-88-909922-0-9

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