About: Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf
Description	In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouring Z-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies. In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouring Z-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies. (en)
Title	Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella (en)
skos:prefLabel	Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella (en)
skos:notation	RIV/60077344:_____/14:00436606!RIV15-GA0-60077344
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0032), P(GA14-22765S), P(GP14-35819P), S
http://linked.open...iv/cisloPeriodika	Supplement 2
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Nguyen, Petr Marec, František Šíchová, Jindra Carabajal Paladino, Leonela Z.
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	27371
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/14:00436606
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cydia pomonella (en)
http://linked.open.../riv/klicoveSlovo	Cydia pomonella
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[CF5AEDD2D051]
http://linked.open...i/riv/nazevZdroje	B M C Genetics
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Promotion of post-doctoral positions in the Biology Centre of the Czech Academy of Sciences aimed to build the global competitiveness of the research teams in the Czech Republic Turnover of sex chromosomes in the karyotype evolution of Lepidoptera Role neo-pohlavních chromosomů v adaptivní radiaci bazálních motýlů skupiny Ditrysia
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Marec, František Šíchová, Jindra Nguyen, Petr Carabajal Paladino, Leonela Z.
issn	1471-2156
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1186/1471-2156-15-S2-S15

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