About: Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Expression of the previously reported defensin of the tick Dermacentor marginatus (defDM) was analysed in different organs by RT-PCR. mRNA of the defDM gene was detected in the hemolymph, midgut and salivary glands. Moreover defDM was isolated from the tick hemolymph using RP-HPLC and its sequence was determined by mass spectrometry and Edman degradation. Synthetic peptide was used for determining biological activities. The results showed an anti-Gram-positive bacterial role for the defensin. As D. marginatus ticks appear not to be vectors of the Lyme disease agent of the complex Borrelia burgdorferi sensu lato, we tested the influence of defDM on Borrelia afzelii. There is a very clear borrelicidal activity of the defensin, which is concentration dependent and suggests a possible role in the clearing of Borrelia ingested by D. marginatus ticks. Expression of the previously reported defensin of the tick Dermacentor marginatus (defDM) was analysed in different organs by RT-PCR. mRNA of the defDM gene was detected in the hemolymph, midgut and salivary glands. Moreover defDM was isolated from the tick hemolymph using RP-HPLC and its sequence was determined by mass spectrometry and Edman degradation. Synthetic peptide was used for determining biological activities. The results showed an anti-Gram-positive bacterial role for the defensin. As D. marginatus ticks appear not to be vectors of the Lyme disease agent of the complex Borrelia burgdorferi sensu lato, we tested the influence of defDM on Borrelia afzelii. There is a very clear borrelicidal activity of the defensin, which is concentration dependent and suggests a possible role in the clearing of Borrelia ingested by D. marginatus ticks. (en)
Title	Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes (en)
skos:prefLabel	Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes (en)
skos:notation	RIV/60077344:_____/14:00435108!RIV15-GA0-60077344
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP302/11/1901)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Grubhoffer, Libor Chrudimská, Tereza Rego, Ryan O. M.
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	10008
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/14:00435108
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Tick; Dermacentor marginatus; Defensin; Borrelia afzelii; Antimicrobial activity; Peptide synthesis (en)
http://linked.open.../riv/klicoveSlovo	Antimicrobial activity Peptide synthesis Tick Borrelia afzelii Defensin Dermacentor marginatus
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[DF4F508CFDC5]
http://linked.open...i/riv/nazevZdroje	Developmental and Comparative Immunology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Antimikrobiální proteiny v imunitním systému kompetentních a nekompetentních vektorů spirochét Borrelia burgdorferi: hledání boréliacidního faktoru.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Grubhoffer, Libor Slaninová, Jiřina Čeřovský, Václav Chrudimská, Tereza Rego, Ryan O. M.
http://linked.open...ain/vavai/riv/wos	000340216600006
issn	0145-305X
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.dci.2014.04.005

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