About: Functional and Complementation Analysis of Hop Genes in Heterologous Systems

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.actahort.org/books/1010/
Description	To investigate the function of transcription factors regulating hop prenylflavonoid biosynthetic pathway in vivo, we cloned the appropriate genes into Agrobacterium tumefaciens vectors under control of constitutive promoter 35S to reach their stable overexpression in plants. Because the hop transformation is labor intensive and time consuming, we performed such analysis firstly on heterologous systems Arabidopsis thaliana, Nicotiana sp. and Petunia hybrida. Overexpression of the hop TF genes showed that they are able to affect plant morphology. To get more knowledge about the hop TFs function, we used known Arabidopsis mutants of genes involved in similar pathways as presumed for hop genes to perform complementation study. The results showed that hop TFs genes are able to regulate the flavonoid and/or anthocyanin biosynthesis pathway. To investigate the function of transcription factors regulating hop prenylflavonoid biosynthetic pathway in vivo, we cloned the appropriate genes into Agrobacterium tumefaciens vectors under control of constitutive promoter 35S to reach their stable overexpression in plants. Because the hop transformation is labor intensive and time consuming, we performed such analysis firstly on heterologous systems Arabidopsis thaliana, Nicotiana sp. and Petunia hybrida. Overexpression of the hop TF genes showed that they are able to affect plant morphology. To get more knowledge about the hop TFs function, we used known Arabidopsis mutants of genes involved in similar pathways as presumed for hop genes to perform complementation study. The results showed that hop TFs genes are able to regulate the flavonoid and/or anthocyanin biosynthesis pathway. (en)
Title	Functional and Complementation Analysis of Hop Genes in Heterologous Systems Functional and Complementation Analysis of Hop Genes in Heterologous Systems (en)
skos:prefLabel	Functional and Complementation Analysis of Hop Genes in Heterologous Systems Functional and Complementation Analysis of Hop Genes in Heterologous Systems (en)
skos:notation	RIV/60077344:_____/13:00426211!RIV14-AV0-60077344
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-03037S)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Matoušek, Jaroslav Kocábek, Tomáš
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	75867
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/13:00426211
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chalconsynthase; transcription factors; Humulus lupulus; Arabidopsis; transgenosis (en)
http://linked.open.../riv/klicoveSlovo	transgenosis Humulus lupulus transcription factors Arabidopsis chalconsynthase
http://linked.open...ontrolniKodProRIV	[C5CD1D19BDB1]
http://linked.open...v/mistoKonaniAkce	Žatec
http://linked.open...i/riv/mistoVydani	Leuven
http://linked.open...i/riv/nazevZdroje	Proceedings of the International Humulus Symposium /3./
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Combinatorial regulation and network of transcription factors involved in the biosynthesis of medicinal prenylflavonoids in hop (Humulus lupulus L.).
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Matoušek, Jaroslav Kocábek, Tomáš
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2012-09-09 (xsd:date)
number of pages	8 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS Acta Horticulturae
https://schema.org/isbn	9789066056961

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