About: Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies

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About: Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A total of 9 (8 stallions and 1 mare) 1 year old ponies were used for the experimental infection caused by Encephalitozoon cuniculi genotype II (107 spores per animal). Subsequently, individual horses were slaughtered 7, 14, 21, 28, 35, 42, 49, 56, and 63 days post infection. Immediately after slaughter, tissues samples of stomach, duodenum, jejunum, ileum, caecum, colon, spleen, liver, kidney, bladder, heart, lungs, and brain were sampled. In addition, urine, feces and blood specimens were collected. Enzyme-linked immunosorbent assay was used for determination of humoral immune response and nested PCR targeting 16S rDNA, whole ITS, and 5.8S rDNA was used for detection of E. cuniculi in collected organs, blood, feces and urine. No clinical signs of microsporidiosis including diarrhea or colic, neurological signs and fever were observed in any horses during whole experiment. Acute microsporidiosis in ponies was characterized by the dissemination of microsporidia into almost all organs and significant increase of concentration of specific antibodies in blood was observed from 28 to 42 DPI. After this acute stage microsporidia disappeared from most organs with the exception of the kidney, which was positive up to 63 DPI when the experiment was terminated. No pathological changes were observed in any organs with exception of one mare's brain, where E. cuniculi-positive cavity measuring 5cm x 3 cm in diameter formed in the lobus pinformis. A total of 9 (8 stallions and 1 mare) 1 year old ponies were used for the experimental infection caused by Encephalitozoon cuniculi genotype II (107 spores per animal). Subsequently, individual horses were slaughtered 7, 14, 21, 28, 35, 42, 49, 56, and 63 days post infection. Immediately after slaughter, tissues samples of stomach, duodenum, jejunum, ileum, caecum, colon, spleen, liver, kidney, bladder, heart, lungs, and brain were sampled. In addition, urine, feces and blood specimens were collected. Enzyme-linked immunosorbent assay was used for determination of humoral immune response and nested PCR targeting 16S rDNA, whole ITS, and 5.8S rDNA was used for detection of E. cuniculi in collected organs, blood, feces and urine. No clinical signs of microsporidiosis including diarrhea or colic, neurological signs and fever were observed in any horses during whole experiment. Acute microsporidiosis in ponies was characterized by the dissemination of microsporidia into almost all organs and significant increase of concentration of specific antibodies in blood was observed from 28 to 42 DPI. After this acute stage microsporidia disappeared from most organs with the exception of the kidney, which was positive up to 63 DPI when the experiment was terminated. No pathological changes were observed in any organs with exception of one mare's brain, where E. cuniculi-positive cavity measuring 5cm x 3 cm in diameter formed in the lobus pinformis. (en)
Title	Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies (en)
skos:prefLabel	Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies Humoral immune response and spreading of Encephalitozoon cuniculi infection in experimentally infected ponies (en)
skos:notation	RIV/60077344:_____/13:00421260!RIV14-AV0-60077344
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Sak, Bohumil Květoňová, Dana Kváč, Martin Wágnerová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	78352
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/13:00421260
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ponies; Encephalitozoon cuniculi genotype II; PCR; Antibodies; Experimental infection (en)
http://linked.open.../riv/klicoveSlovo	PCR Encephalitozoon cuniculi genotype II Ponies Antibodies Experimental infection
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C06ABB16AE6B]
http://linked.open...i/riv/nazevZdroje	Veterinary Parasitology
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	197
http://linked.open...iv/tvurceVysledku	Kváč, Martin Květoňová, Dana Sak, Bohumil Maršálek, M. Wágnerová, Pavla Langerová, I.
http://linked.open...ain/vavai/riv/wos	000324787400001
issn	0304-4017
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.vetpar.2013.05.007

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