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  • To develop a cost-effective biopesticide, spent mushroom substrate (SMS) extract was studied as a potential carbon source for cultivating Bacillus thuringiensis (Bt). Several pretreatments were compared to determine the optimal method for degrading cellulose to produce reducing sugars, including dilute sulfuric acid (0.5–2.0% v/v, 50–121 _C, 1 h), sodium hydroxide (0.5–2% w/v, 50–121 _C, 1 h), calcium hydroxide (0.2–4% w/v, 50–121 _C, 1 h), and hot water (50–121 _C, 1 h). Pretreatment was followed by standard enzymatic hydrolysis and fermentation. Results showed that the highest cellulose degradation was obtained using 2% dilute sulfuric acid pretreatment at 121 _C for 1 h, resulting in a high yield of reducing sugar (284.24 g/kg SMS). Sporulation was also highest using the same pretreatment. Use of SMS is not only an alternative way to commercialize Bt-based biopesticide, but also a potential solution for the environmental pollution associated with accumulation of the spent substrate of the mushroom industry.
  • To develop a cost-effective biopesticide, spent mushroom substrate (SMS) extract was studied as a potential carbon source for cultivating Bacillus thuringiensis (Bt). Several pretreatments were compared to determine the optimal method for degrading cellulose to produce reducing sugars, including dilute sulfuric acid (0.5–2.0% v/v, 50–121 _C, 1 h), sodium hydroxide (0.5–2% w/v, 50–121 _C, 1 h), calcium hydroxide (0.2–4% w/v, 50–121 _C, 1 h), and hot water (50–121 _C, 1 h). Pretreatment was followed by standard enzymatic hydrolysis and fermentation. Results showed that the highest cellulose degradation was obtained using 2% dilute sulfuric acid pretreatment at 121 _C for 1 h, resulting in a high yield of reducing sugar (284.24 g/kg SMS). Sporulation was also highest using the same pretreatment. Use of SMS is not only an alternative way to commercialize Bt-based biopesticide, but also a potential solution for the environmental pollution associated with accumulation of the spent substrate of the mushroom industry. (en)
Title
  • Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar
  • Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar (en)
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  • Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar
  • Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar (en)
skos:notation
  • RIV/60077344:_____/13:00397538!RIV14-AV0-60077344
http://linked.open...avai/riv/aktivita
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  • I
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  • NOV 9
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  • 99097
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  • RIV/60077344:_____/13:00397538
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  • Bacillus thuringiensis; high performace liquid chromatography; pretreatment (en)
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  • NL - Nizozemsko
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  • [487902DAEC57]
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  • Bioresource Technology
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  • 148
http://linked.open...iv/tvurceVysledku
  • Chen, S.
  • Gelbič, Ivan
  • Zhang, L.
  • Wu, S.
  • Wu, Z.
  • Guan, X.
  • Peng, Y.
  • Xu, L.
  • Huang, Z.
  • Lan, Y.
  • Zou, S.
http://linked.open...ain/vavai/riv/wos
  • 000326905400081
issn
  • 0960-8524
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.biortech.2013.08.122
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