About: Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato

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About: Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf
Description	Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms. Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms. (en)
Title	Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato (en)
skos:prefLabel	Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato (en)
skos:notation	RIV/60077344:_____/13:00395286!RIV14-AV0-60077344
http://linked.open...avai/riv/aktivita	I P V
http://linked.open...avai/riv/aktivity	I, P(QI91A229), V
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Habuštová, Oxana Kodrík, Dalibor Sehnal, František Vinokurov, Konstantin Kludkiewicz, Barbara
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	100505
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/13:00395286
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	genetically modified organism; silk protease inhibitor; protease (en)
http://linked.open.../riv/klicoveSlovo	genetically modified organism silk protease inhibitor protease
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[41B7A2675E25]
http://linked.open...i/riv/nazevZdroje	Applied Biochemistry and Biotechnology
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	171
http://linked.open...iv/tvurceVysledku	Horáčková, V. Kodrík, Dalibor Sehnal, František Svobodová, Z. Navrátil, Oldřich Vinokurov, Konstantin Kludkiewicz, Barbara Skoková Habuštová, Oxana
http://linked.open...ain/vavai/riv/wos	000324109800018
issn	0273-2289
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12010-013-0325-9

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