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  • Diatoms represent one of the most important phytoplankton groups. As they grow, diatoms use silicon to produce a siliceous frustule, which protects the cell. In April– November 2011, at 1–3 weeks intervals, we estimated diatom silification rates at two distinct sites along the longitudinal profile of the canyon-shaped eutrophic Římov Reservoir (Czech Republic): (i) a nutrient-depleted lacustrine zone near the dam and (ii) a nutrient-rich transition zone upstream near the river inflow. Diatom silification was estimated using the 24–hour in situ incubation of natural phytoplankton assemblage with a fluorophore 2-(4-pyridyl)-5-{[4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy]phenyl}oxazole (PDMPO) which fluorescently stains the newly synthesized diatom frustules. In the lacustrine zone, diatoms contributed on the average cca 40 % to the total phytoplankton biovolume but only cca 20 % in the transition zone where cyanobacterial bloom developed during summer. Asterionella formosa and Fragilaria crotonensis were the most abundant species, while Aulacoseira italica, Nitzschia acicularis, Synedra acus and Stephanodiscus sp. were far less important. Silification rates of all diatom species were significantly higher in the transition zone than at the dam. The intensity of PDMPO fluorescence per diatom cell was tightly related to growth rates of three diatom species calculated from changes in cell counts during the incubation. The PDMPO technique can thus be used as a proxy for diatom growth. Growth rates of the two most abundant diatom species were positively correlated with daily light exposure but not with nutrient concentrations.
  • Diatoms represent one of the most important phytoplankton groups. As they grow, diatoms use silicon to produce a siliceous frustule, which protects the cell. In April– November 2011, at 1–3 weeks intervals, we estimated diatom silification rates at two distinct sites along the longitudinal profile of the canyon-shaped eutrophic Římov Reservoir (Czech Republic): (i) a nutrient-depleted lacustrine zone near the dam and (ii) a nutrient-rich transition zone upstream near the river inflow. Diatom silification was estimated using the 24–hour in situ incubation of natural phytoplankton assemblage with a fluorophore 2-(4-pyridyl)-5-{[4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy]phenyl}oxazole (PDMPO) which fluorescently stains the newly synthesized diatom frustules. In the lacustrine zone, diatoms contributed on the average cca 40 % to the total phytoplankton biovolume but only cca 20 % in the transition zone where cyanobacterial bloom developed during summer. Asterionella formosa and Fragilaria crotonensis were the most abundant species, while Aulacoseira italica, Nitzschia acicularis, Synedra acus and Stephanodiscus sp. were far less important. Silification rates of all diatom species were significantly higher in the transition zone than at the dam. The intensity of PDMPO fluorescence per diatom cell was tightly related to growth rates of three diatom species calculated from changes in cell counts during the incubation. The PDMPO technique can thus be used as a proxy for diatom growth. Growth rates of the two most abundant diatom species were positively correlated with daily light exposure but not with nutrient concentrations. (en)
Title
  • Spatial heterogeneity of diatom silicification and growth in a eutrophic reservoir
  • Spatial heterogeneity of diatom silicification and growth in a eutrophic reservoir (en)
skos:prefLabel
  • Spatial heterogeneity of diatom silicification and growth in a eutrophic reservoir
  • Spatial heterogeneity of diatom silicification and growth in a eutrophic reservoir (en)
skos:notation
  • RIV/60077344:_____/13:00394771!RIV14-AV0-60077344
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GAP504/11/2177), P(GAP504/11/2182), S
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 106794
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/13:00394771
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • phytoplankton; PDMPO; growth rate; silica deposition; fluorescence (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [085B4454059B]
http://linked.open...i/riv/nazevZdroje
  • Freshwater Biology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 58
http://linked.open...iv/tvurceVysledku
  • Nedoma, Jiří
  • Rychtecký, Pavel
  • Znachor, Petr
  • Visocka, V.
http://linked.open...ain/vavai/riv/wos
  • 000321328800011
issn
  • 0046-5070
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/fwb.12178
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