About: Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene

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About: Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A transformation vector harboring the marker gene aadA conferring spectinomycin resistance and the fusion E7GGG/gus gene of interest was employed for the transformation of tobacco cpDNA via biolistics. The gene of interest consisted of the oncogenic E7GGG gene from human papillomavirus strain 16 fused with the reporter gus gene. We identified 6 plants harboring the E7GGG/gus gene only, where the marker gene was eliminated by homologous recombination, one plant with both transgenes, and one with the aadA gene only. RT-PCR showed the presence of the respective mRNAs in all 8 plants analyzed but Western blot proved that only the GUS part of the fusion E7GGG/GUS protein was present in all plants harboring the fusion gene. A transformation vector harboring the marker gene aadA conferring spectinomycin resistance and the fusion E7GGG/gus gene of interest was employed for the transformation of tobacco cpDNA via biolistics. The gene of interest consisted of the oncogenic E7GGG gene from human papillomavirus strain 16 fused with the reporter gus gene. We identified 6 plants harboring the E7GGG/gus gene only, where the marker gene was eliminated by homologous recombination, one plant with both transgenes, and one with the aadA gene only. RT-PCR showed the presence of the respective mRNAs in all 8 plants analyzed but Western blot proved that only the GUS part of the fusion E7GGG/GUS protein was present in all plants harboring the fusion gene. (en)
Title	Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene (en)
skos:prefLabel	Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene (en)
skos:notation	RIV/60077344:_____/13:00392960!RIV14-AV0-60077344
http://linked.open...avai/riv/aktivita	I P V
http://linked.open...avai/riv/aktivity	I, P(IAA500960903), V
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Vlasák, Josef Bříza, Jindřich Niedermeierová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	111583
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/13:00392960
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	E7GGG oncogene; chloroplast transformation; marker-free plant; homologous recombination (en)
http://linked.open.../riv/klicoveSlovo	homologous recombination chloroplast transformation E7GGG oncogene marker-free plant
http://linked.open...odStatuVydavatele	BG - Bulharská republika
http://linked.open...ontrolniKodProRIV	[451F2A1C3D16]
http://linked.open...i/riv/nazevZdroje	Biotechnology & Biotechnological Equipment
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of a Novel Recombinogenic Technique for Chloroplast Transformation and its Use for Production of human papillomavirus E7 Protein in Plants
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	27
http://linked.open...iv/tvurceVysledku	Vlasák, Josef Bříza, Jindřich Niedermeierová, Hana Ryba, Š. Ludvíková, V.
http://linked.open...ain/vavai/riv/wos	000317997400005
issn	1310-2818
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.5504/BBEQ.2012.0141

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