About: Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed     Goto   Sponge   NotDistinct   Permalink

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Description
  • To explain the association of Bacillus thuringiensis (Bt) with animal feces, an ecological analysis in chickens was conducted by introducing a cry- strain marked by production of green fluorescent protein (GFP). After feeding with the tagged Bt strains, the feces of the tested chickens were collected at different times, isolated, and the morphology of Bt was observed. It was shown that Bt strain HD-73GFP in spore form could be isolated from feces of chickens for a period of 13 d, and then it disappeared thereafter. Bt could be detected only up to day 4 (but not thereafter), when chickens were fed with vegetative cells of HD-73GFP. To confirm the source of newly isolated strains, the gfp gene was examined by polymerase chain reaction (PCR), which showed that all the isolated strains harbored the marker gene. Recent data from isolation and PCR had suggested that fecal Bt strains had originated from food.
  • To explain the association of Bacillus thuringiensis (Bt) with animal feces, an ecological analysis in chickens was conducted by introducing a cry- strain marked by production of green fluorescent protein (GFP). After feeding with the tagged Bt strains, the feces of the tested chickens were collected at different times, isolated, and the morphology of Bt was observed. It was shown that Bt strain HD-73GFP in spore form could be isolated from feces of chickens for a period of 13 d, and then it disappeared thereafter. Bt could be detected only up to day 4 (but not thereafter), when chickens were fed with vegetative cells of HD-73GFP. To confirm the source of newly isolated strains, the gfp gene was examined by polymerase chain reaction (PCR), which showed that all the isolated strains harbored the marker gene. Recent data from isolation and PCR had suggested that fecal Bt strains had originated from food. (en)
Title
  • Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed
  • Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed (en)
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  • Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed
  • Microbiological ecology and association of Bacillus thuringiensis in chicken feces originating from feed (en)
skos:notation
  • RIV/60077344:_____/12:00383105!RIV13-AV0-60077344
http://linked.open...avai/riv/aktivita
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  • P(2B08003), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika
  • 6
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  • 150313
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  • RIV/60077344:_____/12:00383105
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  • Bacillus thuringiensis (en)
http://linked.open.../riv/klicoveSlovo
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  • US - Spojené státy americké
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  • [E04D35D8CBBC]
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  • Current Microbiology
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  • 65
http://linked.open...iv/tvurceVysledku
  • Gelbič, Ivan
  • Zhang, L.
  • Wu, S.
  • Guan, X.
  • Huang, E.
  • Peng, Y.
  • Sun, L.
  • Huang, T.
  • Wu, Ch.
  • Xu, L.
http://linked.open...ain/vavai/riv/wos
  • 000310073500021
http://linked.open...n/vavai/riv/zamer
issn
  • 0343-8651
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s00284-012-0231-3
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