About: MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

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About: MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 %22core'' complex containing five to six proteins, including MRB3010. In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 %22core'' complex containing five to six proteins, including MRB3010. (en)
Title	MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process (en)
skos:prefLabel	MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process (en)
skos:notation	RIV/60077344:_____/11:00359407!RIV12-MSM-60077344
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(2B06129), P(GA204/09/1667), P(LC07032), S, Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Lukeš, Julius Hashimi, Hassan Číčová, Zdeňka Novotná, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	214138
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00359407
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	RNA editing; trypanosome; MRB1 complex; mitochondria; kinetoplast (en)
http://linked.open.../riv/klicoveSlovo	RNA editing MRB1 complex kinetoplast trypanosome mitochondria
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2DC3E2DBD042]
http://linked.open...i/riv/nazevZdroje	R N A
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Model organisms as a key to the understanding of functions of disease-related human genes Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei Center for Functional Genetics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Lukeš, Julius Hashimi, Hassan Ammerman, M. L. Mcevoy, S. M. Novotná, Lucie Read, L. K. Číčová, Zdeňka
http://linked.open...ain/vavai/riv/wos	000289617400009
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	1355-8382
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1261/rna.2446311

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