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Description
| - Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni.
- Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. (en)
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Title
| - Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni
- Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni (en)
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skos:prefLabel
| - Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni
- Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni (en)
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skos:notation
| - RIV/60077344:_____/11:00358458!RIV12-AV0-60077344
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/11:00358458
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - ASTERN UNITED-STATES; RIBOSOMAL-RNA GENE; PHYLOGENETIC ANALYSIS; MOLECULAR ANALYSIS; NATURAL INFECTION; DAIRY-CATTLE; PREVALENCE; GENOTYPES; HUMANS; KENYA (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Clinical Microbiology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kváč, Martin
- Li, N.
- Modrý, David
- Fayer, R.
- Feng, Y.
- Koudela, Břetislav
- Ryan, U. M.
- Xiao, L.
- Yang, W.
- Zhang, L.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://bibframe.org/vocab/doi
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