About: Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

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About: Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg, whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom. We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg, whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom. (en)
Title	Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families (en)
skos:prefLabel	Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families (en)
skos:notation	RIV/60077344:_____/11:00358401!RIV12-AV0-60077344
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA522/09/1940), P(KJB501410901), S, Z(AV0Z50070508), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Starý, Petr Havelka, Jan Vítková, Magda Novotná, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	207200
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00358401
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Diuraphis noxia; karyotype; genome size (en)
http://linked.open.../riv/klicoveSlovo	genome size Diuraphis noxia karyotype
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[DB1EA80D55FB]
http://linked.open...i/riv/nazevZdroje	Genetica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Population genetics and environmental interactions of Russian wheat aphid Diuraphis noxia, an alien invasive pest of cereals in the Czech republic Analysis of sequences and distribution of the selfish DNA in the genome of moths and butterflies (Lepidoptera).
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	139
http://linked.open...iv/tvurceVysledku	Havelka, Jan Starý, Petr Novotná, Jana Koutecký, P. Vítková, Magda
http://linked.open...ain/vavai/riv/wos	000288514100001
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	0016-6707
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10709-011-9546-4

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