About: The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei

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About: The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fe/S clusters are part of the active site of many enzymes and are essential for cell viability. In eukaryotes the cysteine desulfurase Nfs (IscS) donates the sulfur during Fe/S cluster assembly and was thought sufficient for this reaction. Moreover, Nfs is indispensable for tRNA thiolation, a modification generally required for tRNA function and protein synthesis. Recently, Isd11 was discovered as an integral part of the Nfs activity at an early step of Fe/S cluster assembly. Here we show, using a combination of genetic, molecular, and biochemical approaches, that Isd11, in line with its strong association with Nfs, is localized in the mitochondrion of T. brucei. In addition to its involvement in Fe/S assembly, Isd11 also partakes in both cytoplasmic and mitochondrial tRNA thiolation, whereas Mtu1, another protein proposed to collaborate with Nfs in tRNA thiolation, is required for this process solely within the mitochondrion. Fe/S clusters are part of the active site of many enzymes and are essential for cell viability. In eukaryotes the cysteine desulfurase Nfs (IscS) donates the sulfur during Fe/S cluster assembly and was thought sufficient for this reaction. Moreover, Nfs is indispensable for tRNA thiolation, a modification generally required for tRNA function and protein synthesis. Recently, Isd11 was discovered as an integral part of the Nfs activity at an early step of Fe/S cluster assembly. Here we show, using a combination of genetic, molecular, and biochemical approaches, that Isd11, in line with its strong association with Nfs, is localized in the mitochondrion of T. brucei. In addition to its involvement in Fe/S assembly, Isd11 also partakes in both cytoplasmic and mitochondrial tRNA thiolation, whereas Mtu1, another protein proposed to collaborate with Nfs in tRNA thiolation, is required for this process solely within the mitochondrion. (en)
Title	The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei (en)
skos:prefLabel	The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei (en)
skos:notation	RIV/60077344:_____/10:00347296!RIV11-GA0-60077344
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA204/09/1667), P(LC07032), S, Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	29
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Lukeš, Julius Paris, Zdeněk Zíková, Alena Changmai, Piya
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	259020
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/10:00347296
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	IRON-SULFUR PROTEINS; SACCHAROMYCES-CEREVISIAE; CYSTEINE DESULFURASE; THIO-MODIFICATION; FRATAXIN (en)
http://linked.open.../riv/klicoveSlovo	FRATAXIN SACCHAROMYCES-CEREVISIAE CYSTEINE DESULFURASE IRON-SULFUR PROTEINS THIO-MODIFICATION
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B75D172FCA40]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei Center for Functional Genetics
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	285
http://linked.open...iv/tvurceVysledku	Alfonzo, J. D. Lukeš, Julius Paris, Zdeněk Changmai, Piya Stuart, K. D. Zíková, Alena RUBIO, M. A. T.
http://linked.open...ain/vavai/riv/wos	000279702200052
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	0021-9258
number of pages	9 (xsd:int)

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