About: Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Daphnia galeata, D. longispina and D. cucullata are closely related species which often produce interspecific hybrids. Several marker systems are available for taxon determination in this hybridizing complex, but their performance and reliability has not been systematically assessed. We compared results from identifications by three molecular methods. More than 1,200 individuals from 10 localities in the Czech Republic were identified as parental species or hybrids by allozyme electrophoresis and the analysis of the restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP); over 440 of them were additionally analyzed and identified by 12 microsatellite loci. Identification by microsatellite markers corresponded well with allozyme analyses. However, consistent discrepancies between ITS-RFLP and other markers were observed in two out of 10 studied localities. These results warn against its use as a sole identification method in field studies. Daphnia galeata, D. longispina and D. cucullata are closely related species which often produce interspecific hybrids. Several marker systems are available for taxon determination in this hybridizing complex, but their performance and reliability has not been systematically assessed. We compared results from identifications by three molecular methods. More than 1,200 individuals from 10 localities in the Czech Republic were identified as parental species or hybrids by allozyme electrophoresis and the analysis of the restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP); over 440 of them were additionally analyzed and identified by 12 microsatellite loci. Identification by microsatellite markers corresponded well with allozyme analyses. However, consistent discrepancies between ITS-RFLP and other markers were observed in two out of 10 studied localities. These results warn against its use as a sole identification method in field studies. (en)
Title	Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches (en)
skos:prefLabel	Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches (en)
skos:notation	RIV/60077344:_____/10:00343446!RIV11-GA0-60077344
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA206/04/0190), P(GEDIV/06/E007), P(IAA600960901), S, Z(AV0Z60170517), Z(MSM0021620828)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Seďa, Jaromír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	262769
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/10:00343446
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Daphnia; interspecific hybridization; internal transcribed spacer; allozyme electrophoresis; microsatellites (en)
http://linked.open.../riv/klicoveSlovo	internal transcribed spacer microsatellites Daphnia allozyme electrophoresis interspecific hybridization
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[F5403A6E35DE]
http://linked.open...i/riv/nazevZdroje	Hydrobiologia
http://linked.open...in/vavai/riv/obor	DA
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Connectivity, dispersal and priority effects as drivers of biodiversity and ecosystem function in pond and pool communities (BIOPOOL) Local genetic differentiation of Daphnia in deep canyon-shaped dam reservoirs Hybrid zones in pelagic environments: which factors are critical for local dominance of Daphnia hybrids within reservoirs?
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	643
http://linked.open...iv/tvurceVysledku	Petrusek, A. Seďa, Jaromír Schwenk, K. Dlouhá, Š. Kraus, R.H.S. Thielsch, A.
http://linked.open...ain/vavai/riv/wos	000276256500014
http://linked.open...n/vavai/riv/zamer	Struktura, funkce a vývoj vodních ekosystémů Ekologické procesy v evoluci modelových skupin organismů
issn	0018-8158
number of pages	16 (xsd:int)

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