About: Probing for primary functions of prohibitin in Trypanosoma brucei

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes. We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes. (en)
Title	Probing for primary functions of prohibitin in Trypanosoma brucei Probing for primary functions of prohibitin in Trypanosoma brucei (en)
skos:prefLabel	Probing for primary functions of prohibitin in Trypanosoma brucei Probing for primary functions of prohibitin in Trypanosoma brucei (en)
skos:notation	RIV/60077344:_____/10:00342888!RIV11-GA0-60077344
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA204/09/1667), P(GP204/06/P423), P(IAA500960705), P(LC07032), S, Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Týč, Jiří Jirků, Milan Vávrová, Zuzana Lukeš, Julius Kriegová, Eva Faktorová, Drahomíra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	282112
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/10:00342888
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	prohibitin; mitochondrion; morphology; mitochondrial translation (en)
http://linked.open.../riv/klicoveSlovo	mitochondrion morphology mitochondrial translation prohibitin
http://linked.open...odStatuVydavatele	AU - Australské společenství
http://linked.open...ontrolniKodProRIV	[662267DBE845]
http://linked.open...i/riv/nazevZdroje	International Journal for Parasitology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Characterization of a novel protein complex involved in RNA processing and editing in the mitochondrion of Trypanosoma brucei Center for Functional Genetics Functional analysis of prohibitin in the primitive flagellate Trypanosoma brucei The role of Trypanosoma brucei RNA binding proteins in mitochondrial transcript processing.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	40
http://linked.open...iv/tvurceVysledku	Jirků, Milan Kriegová, Eva Lukeš, Julius Vávrová, Zuzana Faktorová, Drahomíra Maslov, D. A. Týč, Jiří
http://linked.open...ain/vavai/riv/wos	000274104300008
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	0020-7519
number of pages	11 (xsd:int)

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