About: GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column

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About: GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Heptafluorobutyl chloroformate, a recently introduced derivatization reagent, was examined in enantioseparation of amino acids by gas chromatography. Twenty proteinogenic amino acids, plus ornithine, cystine and 4-fluorophenylalanine (internal standard) were treated with the reagent and separation properties of the derivatives assessed on a Chirasil-Val capillary column. Nineteen amino acid enantiomers were efficiently separated in 43 minutes except proline, arginine and cystine. The heptafluorobutyl chloroformate derivatives of the studied DL-amino acids show improved separation over other chloroformate-based derivatives hitherto reported. A combination of the improved and faster separation with a simple derivatization protocol, involving an immediate one-step reaction-extraction in two-phase aqueous-organic medium, and low elution temperatures extend application of heptafluorobutyl chloroformate to chiral amino acid analysis. Heptafluorobutyl chloroformate, a recently introduced derivatization reagent, was examined in enantioseparation of amino acids by gas chromatography. Twenty proteinogenic amino acids, plus ornithine, cystine and 4-fluorophenylalanine (internal standard) were treated with the reagent and separation properties of the derivatives assessed on a Chirasil-Val capillary column. Nineteen amino acid enantiomers were efficiently separated in 43 minutes except proline, arginine and cystine. The heptafluorobutyl chloroformate derivatives of the studied DL-amino acids show improved separation over other chloroformate-based derivatives hitherto reported. A combination of the improved and faster separation with a simple derivatization protocol, involving an immediate one-step reaction-extraction in two-phase aqueous-organic medium, and low elution temperatures extend application of heptafluorobutyl chloroformate to chiral amino acid analysis. (en)
Title	GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column (en)
skos:prefLabel	GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column (en)
skos:notation	RIV/60077344:_____/09:00335962!RIV10-AV0-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/09/2014), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Šimek, Petr Zahradníčková, Helena Hušek, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	315993
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/09:00335962
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chiral separation; derivatization; D,L-AAs (en)
http://linked.open.../riv/klicoveSlovo	L-AAs chiral separation derivatization D
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[3B2EBD2D5A06]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	New approaches to alkylchloroformate-mediated sample preparation for parallel GC-MS and LC-MS for metabolite analysis in biofluids
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Hušek, Petr Šimek, Petr Zahradníčková, Helena
http://linked.open...ain/vavai/riv/wos	000273993500008
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems
issn	1615-9306
number of pages	6 (xsd:int)

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